Pharmaceutisch Weekblad

, Volume 2, Issue 1, pp 557–561 | Cite as

Rapid and selective theophylline serum and saliva assay by means of high pressure liquid chromatography

  • J. H. G. Jonkman
  • R. Schoenmaker
  • J. E. Greying
  • R. A. de Zeeuw
Original Articles


A rapid, selective and sensitive high pressure liquid Chromatographic (HPLC) method for the determination of theophylline in human serum (or plasma) and saliva was developed.

When using 0.5 ml of serum, concentrations down to 0.4 mg.l−1 could be accurately measured. Each sample requires only about 15 minutes for the completion of the assay, including sample preparation. The actual chromatography time is about 8 minutes. The theophylline metabolites and other xanthines, as theobromine, caffeine (and its metabolite paraxanthine) are well separated. The sensitivity, precision and accuracy are sufficient for routine monitoring of therapeutic theophylline serum levels in patients (approximately 10 to 20 mg.l−1). Reliability of the method was demonstrated during analysis of about 3000 samples on the same column.


Liquid Chromatography Serum Level Caffeine Theophylline Human Serum 
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Copyright information

© Bohn, Scheltema & Holkema 1980

Authors and Affiliations

  • J. H. G. Jonkman
    • 1
    • 2
  • R. Schoenmaker
    • 2
  • J. E. Greying
    • 1
  • R. A. de Zeeuw
    • 1
  1. 1.Department of Pharmaceutical and Analytical ChemistryUniversity of GroningenAW GroningenThe Netherlands
  2. 2.Laboratory for Drug AnalysisGW AssenThe Netherlands

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