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Pharmaceutisch weekblad

, Volume 3, Issue 1, pp 582–586 | Cite as

HPLC-estimation of tobramycin in serum

  • P. G. L. C. Krugers Dagneaux
  • J. T. Klein Elhorst
Original Articles

Abstract

This paper describes a method for estimating tobramycin in serum with reversed phase high-pressure liquid chromatography (HPLC) and fluorescence detection. Sisomicin as an internal standard, and perchloric acid are added to the serum. After centrifuging, the clear supernatant is directly injected onto a Lichrosorb RP-18 column. The elution is performed by means of a gradient of two solvents. Postcolumn the eluate is mixed continuously with ano-phthalaldehyde reagent and the fluorescence is measured. Good correlation is obtained with aRia-method and a classic microbiological estimation method. The method is suitable for clinical use in routine laboratories.

Keywords

Quinidine Tobramycin Disopyramide Sisomicin Reaction Coil 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Bohn, Scheltema & Holkema 1981

Authors and Affiliations

  • P. G. L. C. Krugers Dagneaux
    • 1
  • J. T. Klein Elhorst
    • 1
  1. 1.Ziekenhuis De StadsmatenJX EnschedeThe Netherlands

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