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Pharmaceutisch Weekblad

, Volume 4, Issue 1, pp 21–24 | Cite as

Separation of valtrate and isovaltrate by means of preparative liquid chromatography

  • B. Hazelhoff
  • R. Jellema
  • H. Grobben
  • TH. M. Malingré
Short Communications

Abstract

In this study valtrate and isovaltrate, two isomeric valepotriates present in variousValeriana species, were separated on a preparative scale with a Waters preparative Liquid Chromatography/500 a system. A rapid separation was achieved with the solvent methylene chloriden-propanol-acetone (99 + 0.5 + 0.5). Extensive use of the recycle mode of the instrument resulted in a considerable reduction of the amount of solvent used, without affecting the purity and the recovery (> 90%) of the separated components. With the method presented in this communication 1 gram of a mixture of valtrate and isovaltrate was separated in 20 minutes using 2.1 liters of solvent.

Keywords

Public Health Methylene Internal Medicine Liquid Chromatography Considerable Reduction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1982

Authors and Affiliations

  • B. Hazelhoff
    • 1
  • R. Jellema
    • 1
  • H. Grobben
    • 1
  • TH. M. Malingré
    • 1
  1. 1.Laboratory for PharmacognosyState UniversityAW GroningenThe Netherlands

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