Separation of valtrate and isovaltrate by means of preparative liquid chromatography
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In this study valtrate and isovaltrate, two isomeric valepotriates present in variousValeriana species, were separated on a preparative scale with a Waters preparative Liquid Chromatography/500 a system. A rapid separation was achieved with the solvent methylene chloriden-propanol-acetone (99 + 0.5 + 0.5). Extensive use of the recycle mode of the instrument resulted in a considerable reduction of the amount of solvent used, without affecting the purity and the recovery (> 90%) of the separated components. With the method presented in this communication 1 gram of a mixture of valtrate and isovaltrate was separated in 20 minutes using 2.1 liters of solvent.
KeywordsPublic Health Methylene Internal Medicine Liquid Chromatography Considerable Reduction
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