Pharmaceutisch Weekblad

, Volume 7, Issue 2, pp 79–82 | Cite as

Colorimetric analysis of some diuretic drugs: hydrochlorothiazide and spironolactone

  • Bahia A. Moussa
  • Naglaa M. el Kousy
Short Communications


Two convenient spectrophotometric methods were developed for the determination of hydrochlorothiazide and spironolactone. A specific colour reaction for the determination of hydrochlorothiazide is reported. One method is based on the reaction of hydrochlorothiazide withn-butylamine and cobaltous chloride in anhydrous methanol. A blue-violet colour is produced and is measured at 570 nm. A highly selective colorimetric method was adopted for the analysis of spironolactone by reacting with isoniazid forming a coloured hydrazone derivative and subsequent measurement of the coloured product at 405 nm.


Public Health Chloride Methanol Internal Medicine Isoniazid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Dreux C, Halter D. Spectrofluorimetric determination of furosemide in biological fluids. Ann Biol Clin 1976;34(2):113–20.Google Scholar
  2. 2.
    Daniels SL, Vanderwielen AJ. Stability-indicating assay of hydrochlorothiazide. J Pharm Sci 1981; 70(2):211–5.PubMedGoogle Scholar
  3. 3.
    British Pharmacopoia. London: The Pharmaceutical Press, 1980;224:776.Google Scholar
  4. 4.
    Pharmacopeia of the United States of America xx. Mac Publishing Ci. Easton, 1980:386,740,774.Google Scholar
  5. 5.
    Dessouky YM. Gad El Rub LN. Colour reaction of dichlorphenamide and related sulphonamide drugs. Pharm Weekbl [Sci] 1980:2:124–6.Google Scholar
  6. 6.
    Bult A. The cobaltous amine reaction. II. Cobalt complexes of barbiturates, methimazole, methylthiouracil, naphazoline, phenytoin. sulfathiazole and theophylline. Pharm Weekbl 1976;111:385.Google Scholar
  7. 7.
    British Pharmacopoeia. London: The Pharmaceutical Press, 1963:367–8.Google Scholar
  8. 8.
    British Pharmacopoeia. London: The Pharmaceutical Press, 1973:442Google Scholar

Copyright information

© Bohn, Scheltema & Holkema 1985

Authors and Affiliations

  • Bahia A. Moussa
    • 1
  • Naglaa M. el Kousy
    • 2
  1. 1.Pharmaceutical Chemistry Department, Faculty of PharmacyCairo UniversityCairoEgypt
  2. 2.National Organization of Drug Control and ResearchCairoEgypt

Personalised recommendations