Pharmaceutisch Weekblad

, Volume 7, Issue 4, pp 163–166 | Cite as

Determination of ethinyloestradiol in the presence of norethisterone by derivative-difference spectrophotometry

  • M. A. Korany
  • F. A. El-Yazbi
  • Omyama Abdel-Razak
  • M. A. Elsayed
Short Communications


A simple and direct method for the determination of ethinyloestradiol as a minor component in the presence of norethisterone and in oral contraceptive tablets is presented. The method is based on measuring the signal intensity, dδA/dλ and d2δA/dλ2, of the generated first and second derivative spectra of the δ-absorbance curves obtained by measuring solutions in methanol and methanol-sodium hydroxide at certain wavelengths. The method has been applied to the determination of ethinyloestradiol in oral contraceptive tablets, with a coefficient of variation of less than 2%.


Public Health Methanol Internal Medicine Hydroxide Signal Intensity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Hirai S, Hussain A, Babhair S. Determination of Ethinyloestradiol in presence of Norethisterone fluorimetry. J Pharm Sci 1980;69:857–9.PubMedGoogle Scholar
  2. 2.
    Chen W, Rong D, Sun S. Spectrofluorimetric determination of Ethinyloestradiol in oral contraceptive tablets. Shanghai Diyi Yixueyuan Xuebao 1982;9:339–43, through Anal Abstr 1983;45:2E 28.Google Scholar
  3. 3.
    Johnston MA. General method for assay of oral contraceptive by HPLC. J Chromatogr 1981;216:269–78.CrossRefGoogle Scholar
  4. 4.
    Amin M, Hassen BM. Determination of Ethinyloestradiol in oral contraceptive by t.l.c. fluorimetry (or reflect spectrophotometry). Analyst 1979;104:407–11.CrossRefGoogle Scholar
  5. 5.
    Wu Jhon YP. Colorimetric determination of ethinyloestradiol in oral contraceptive formulation. J Assoc Off Anal Chem 1974;57:747–51.PubMedGoogle Scholar
  6. 6.
    Eldawy MA, Tawfik AS, Elshaboury SR. Rapid sensitive colorimetric method for determination of ethinyloestradiol. J Pharm Sci 1975;64:1221–3.PubMedGoogle Scholar
  7. 7.
    Corti P, Leucioni E, Sciarra GF. Use of derivative resolution for the spectrophotometric determination of drug mixture: III-second derivative determination of binary mixture of oestrogens and progestogens. Boll Chim Farm 1983;122:281–8, through Chem Abstr 1984;100:12744S.Google Scholar
  8. 8.
    Ebel S, Bender R, Weyandt M, Widjaja N. UV photometric determination of Ethinyloestradiol. Dtsch Apot Ztg 1983;123:2151–6, through Chem Abstr 1984;100:12756X.Google Scholar
  9. 9.
    Fell AF. Analysis of pharmaceutical dosage forms by second derivative ultraviolet-visible spectrophotometry. Proc Anal Div Chem Soc 1978;15:260–7.Google Scholar
  10. 10.
    Korany MA, Wahbi MA, Elsayed MA, Mandour S. First derivative spectrophotometric determination of certain drugs in two-component mixtures. Anal Lett 1984;17(B12):1373–89.Google Scholar
  11. 11.
    Fell AF. Biomedical applications of derivative spectroscopy. Trends Anal Chem 1983;2:63–6.CrossRefGoogle Scholar
  12. 12.
    Talsky G, Mayring L, Kreuzer H. Higher-order derivative spectrophotometry for the fine resolution of ultraviolet-visible spectra. Angew Chem [Engl] 1978;17:532–3.CrossRefGoogle Scholar

Copyright information

© Bohn, Scheltema & Holkema 1985

Authors and Affiliations

  • M. A. Korany
    • 1
  • F. A. El-Yazbi
    • 1
  • Omyama Abdel-Razak
    • 1
  • M. A. Elsayed
    • 1
  1. 1.Faculty of Pharmacy, Department of Pharmaceutical Analytical ChemistryAlexandria UniversityAlexandriaEgypt

Personalised recommendations