Pharmaceutisch Weekblad

, Volume 7, Issue 4, pp 150–157 | Cite as

Determination of some anticonvulsants, antiarrhythmics, benzodiazepines, xanthines, paracetamol and chloramphenicol by reversed phase HPLC

  • H. J. J. Willems
  • A. van der Horst
  • P. N. F. C. de Goede
  • G. J. Haakmeester
Original Articles


In this article assays are described for caffeine, theophylline, procainamide,N-acetylprocainamide, quinidine, dihydroquinidine, paracetamol, phenobarbital, phenytoin, carbamazepine, chloramphenicol, oxazepam, temazepam, diazepam, desmethyldiazepam, chlordiazepoxide, desmethylchlordiazepoxide and demoxepam using a uniform working procedure, five (slightly) different mobile phases and one HPLC system. Changing from one eluent to another is simple and a stable base-line is achieved within half an hour. Three of the five eluents are interchangeable and recycling of eluent causes no problems. Sample pretreatment is a single step extraction. Interferences can be overcome by changing the selectivity of the eluent by adjusting the tetrahydrofuran or triethylamine content. Furthermore it is shown how triethylamine can improve peak shapes of basic components and shorten their retention times.


Caffeine Theophylline Carbamazepine Paracetamol Chloramphenicol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Bohn, Scheltema & Holkema 1985

Authors and Affiliations

  • H. J. J. Willems
    • 1
  • A. van der Horst
    • 1
  • P. N. F. C. de Goede
    • 1
  • G. J. Haakmeester
    • 1
  1. 1.Laboratory for Drugs Research, Pharmacy DepartmentUniversity Hospital of the Free University of AmsterdamMB AmsterdamThe Netherlands

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