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Pharmaceutisch Weekblad

, Volume 5, Issue 6, pp 319–324 | Cite as

Determination of formation constants of copper(II) complexes of Adler medium components with a solid-state copper(II) ionselective electrode

  • J. Ch Eriks
  • H. -D. Gaisser
  • H. Van Der Goot
  • H. Timmerman
Original Articles

Abstract

A general method for the determination of overall conditional formation constants of copper(II) complexes of mycoplasma growth medium (Adler) constituents together with the protonation constants of the complexing ligands was developed. In Adler medium a total of 0.204±0.004 molar copper binding sites proved to be present with log β2=8.14±0.05 (μ= 0.11) and log Ka=6.14±0.004. Conditional overall formation constants were calculated at various pH values. Free copper(II) ion concentrations were calculated as a function of the total added amount of copper(II) and pH. The consequences of these findings for the determination of the growth inhibition of mycoplasmas by copper complexes of 2,2′-bipyridyl analogues are stressed.

Keywords

Copper Binding Site Growth Medium Growth Inhibition Copper Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1983

Authors and Affiliations

  • J. Ch Eriks
    • 1
  • H. -D. Gaisser
    • 1
  • H. Van Der Goot
    • 1
  • H. Timmerman
    • 1
  1. 1.Department of PharmacochemistryFree UniversityHV AmsterdamThe Netherlands

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