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Pharmaceutisch Weekblad

, Volume 5, Issue 6, pp 319–324 | Cite as

Determination of formation constants of copper(II) complexes of Adler medium components with a solid-state copper(II) ionselective electrode

  • J. Ch Eriks
  • H. -D. Gaisser
  • H. Van Der Goot
  • H. Timmerman
Original Articles

Abstract

A general method for the determination of overall conditional formation constants of copper(II) complexes of mycoplasma growth medium (Adler) constituents together with the protonation constants of the complexing ligands was developed. In Adler medium a total of 0.204±0.004 molar copper binding sites proved to be present with log β2=8.14±0.05 (μ= 0.11) and log Ka=6.14±0.004. Conditional overall formation constants were calculated at various pH values. Free copper(II) ion concentrations were calculated as a function of the total added amount of copper(II) and pH. The consequences of these findings for the determination of the growth inhibition of mycoplasmas by copper complexes of 2,2′-bipyridyl analogues are stressed.

Keywords

Copper Binding Site Growth Medium Growth Inhibition Copper Complex 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Pijper PJ. Synthesis and antimycoplasmal activity of compounds structurally related to 2,2′-bipyridyl. Amsterdam: Free University, 1980. Ph.D. Thesis.Google Scholar
  2. 2.
    Antić BM, Van der Goot H, Nauta WTh, et al. The influence of copper ions on the growth-inhibitory effect of 2,2′-bipyridyl and related compounds on mycoplasma. Eur J Med Chem 1977;12:573–5.Google Scholar
  3. 3.
    Antić BM, Van der Goot H, Nauta WTh, et al. The influence of copper ions on the growth-inhibitory effect of 2,2′-bipyridyl and related compounds on mycoplasma. Part II. Eur J Med Chem 1978;13:565–8.Google Scholar
  4. 4.
    Smit H. Studies on the mode of action of the copper(I) complex of 2,9-dimethyl-1,10-phenanthroline onParacoccus denitrificans andMycoplasma gallisepticum. Amsterdam: Free University, 1982. Ph.D. Thesis.Google Scholar
  5. 5.
    Smit H, Van der Goot H, Nauta WTh, et al. Studies on the mechanism of action of the copper(I) complex of 2,9-dimethyl-1,10-phenanthroline onMycoplasma gallisepticum. Antimicrob Agents Chemother 1982;21:881–6.Google Scholar
  6. 6.
    Hartley FR, Burgess C, Alcock RM. Solution equilibria. Chichester: John Wiley & Sons, Ltd., 1980.Google Scholar
  7. 7.
    Irving HM, Rosotti HS. The calculation of formation curves of metal complexes from pH titration curves in mixed solvents. J Chem Soc 1954:2904–10.Google Scholar
  8. 8.
    Hansen EH, Lamm CG, Ruzicka J. Selectrode® — The universal ion-selective solid-state electrode. Anal Chim Acta 1972;59:403–26.Google Scholar
  9. 9.
    Wada H, Fernando Q. Determination of formation constants of copper(II) complexes of ethane-1-hydroxy-1,1-diphosphonic acid with a solid-state cupric ion-selective electrode. Anal Chem 1971;43:751–5.Google Scholar
  10. 10.
    Schmid RW, Reilly CN. A rapid electrochemical method for the determination of metal chelate stability constants. J Am Chem Soc 1956;78:5513–8.Google Scholar
  11. 11.
    Vogel AI. A textbook of quantitative inorganic analysis, 3rd ed. London: Longmans, Green and Co., Ltd., 1961:441.Google Scholar
  12. 12.
    Leden I. Einige potentiometrische Messungen zur Bestimmung der Komplex-ionen in Cadmiumsalz Lösungen. Z Physik Chem 1941;A 188:160–81.Google Scholar
  13. 13.
    Margerum DW. Metal peptide complexes. Pure Appl Chem 1983;55(1):23–34.Google Scholar
  14. 14.
    Margerum DW, Dukes R. Kinetics and mechanisms of metal-ion and proton transfer reactions of oligopeptide complexes. In: Sigel H, ed. Metal ions in biological systems. New York: Marcel Dekker Inc., 1974:157–212.Google Scholar
  15. 15.
    Schwarzenbach G. Complexometric titrations (translation Irving HMN). London: Methuen.Google Scholar
  16. 16.
    Ringbom A. The analyst and the inconstant constants. J Chem Education 1958;35:282–8.Google Scholar
  17. 17.
    Alcock RM, Hartley FR, Rogers DE. A damped non-linear least-squares computer program (DALSFEK) for the evaluation of equilibrium constants from spectrophotometric and potentiometric data. J Chem Soc (Dalton) 1978:115–23.Google Scholar
  18. 18.
    Smit H, Van der Goot H, Nauta WTh, et al. Mode of action of the copper(I) complex of 2,9-dimethyl-1,10-phenanthroline onMycoplasma gallisepticum. Antimicrob Agents Chemother 1981;20:455–62.Google Scholar
  19. 19.
    Eriks JCh, et al. Unpublished observations.Google Scholar
  20. 20.
    Stünzi H. Copper(I) activity of the copper(II) ion-selective electrode. Talanta 1982;29:75–6.Google Scholar
  21. 21.
    Oxoid. The Oxoid manual. 3rd ed. London: Oxoid Ltd., 1975.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1983

Authors and Affiliations

  • J. Ch Eriks
    • 1
  • H. -D. Gaisser
    • 1
  • H. Van Der Goot
    • 1
  • H. Timmerman
    • 1
  1. 1.Department of PharmacochemistryFree UniversityHV AmsterdamThe Netherlands

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