Pharmaceutisch Weekblad

, Volume 5, Issue 6, pp 287–290 | Cite as

Do evacuated blood collection tubes interfere with therapeutic drug monitoring?

  • R. Janknegt
  • J. J. H. M. Lohman
  • P. M. Hooymans
  • F. W. H. M. Merkus
Original Articles


The influence of various brands of evacuated blood collection systems (the old type, red stoppered Vacutainer®; the new type, blue stoppered Vacutainer®; Monoject® and Venoject®) on therapeutic drug monitoring was investigated. No interferences were found in the assay of ethosuximide, phenobarbital, phenytoin, valproic acid, digitoxin, digoxin, procainamide, gentamicin and theophylline.

Using Monoject® and old type Vacutainer® tubes, lower levels were found in the disopyramide assay: 91.3±4.6% (p<0.05) and 91.7±7.0% (not significant) respectively, and in the quinidine assay: 82.8±6.7% (p<0.02) and 83.9±4.4% (p<0.001) respectively as compared with glass tubes. In the carbamazepine assay a decrease was found in the Monoject® tubes only: 93.7±1.7% (p<0.01). The stoppers of Monoject® tubes and the old type Vacutainer® tubes contained the plasticizer tris(2-butoxyethyl)phosphate (tbep), which has been shown to be a potent inhibitor of the binding of several drugs to α1-acid glycoprotein.

Using the new type Vacutainer® and the Venoject®, no interferences were found.


Digoxin Gentamicin Theophylline Carbamazepine Valproic Acid 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1983

Authors and Affiliations

  • R. Janknegt
    • 1
  • J. J. H. M. Lohman
    • 1
  • P. M. Hooymans
    • 1
  • F. W. H. M. Merkus
    • 1
    • 2
  1. 1.Department of Clinical PharmacyRoman Catholic Hospital SittardBK SittardThe Netherlands
  2. 2.Department of BiopharmaceuticsUniversity of AmsterdamTV AmsterdamThe Netherlands

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