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Experientia

, Volume 36, Issue 1, pp 139–141 | Cite as

An improved method for the isolation of eosinophilic leukocytes from normal human blood

  • P. Portmann
  • P. Köppel
  • A. Jörg
Pro Experimentis

Summary

A relatively short and simple method for the isolation of the eosinophils from normal human blood is reported. With a recovery of about 45–55%, cell preparations, showing a degree of purity of 90–98%, are obtained. The isolated cells are morphologically intact and viable, as assessed by the trypan blue exclusion test and by active phagocytosis.

Keywords

Trypan Blue Human Blood Improve Method Trypan Blue Exclusion Cell Preparation 

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Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • P. Portmann
    • 1
  • P. Köppel
    • 1
  • A. Jörg
    • 1
  1. 1.Physiologisch-chemisches InstitutUniversität FreiburgFreiburgSwitzerland

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