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Experientia

, Volume 36, Issue 1, pp 124–125 | Cite as

Detection of human ADCC activity using automated flow cytometry

  • A. M. Attallah
  • P. D. Noguchi
  • T. Folks
  • C. T. Noguchi
Specialia

Summary

Using automated flow cytometry techniques we have developed a rapid assay to measure human antibody-dependent cell-mediated cytotoxicity (ADCC). By staining cell cultures for DNA content, chick red blood cell targets can be readily distinguished from human effector cells and ADCC can be measured by changes in their relative proportions. The sensitivity and rapidity of the assay is shown by the finding that at an effector to target ratio of only 2:1, 42% killing can be detected after 1 h incubation.

Keywords

Cell Culture Blood Cell Flow Cytometry Cell Target Effector Cell 

References

  1. 1.
    Acknowledgment. The opinions and assertions contained herein are the private ones of the writers and are not to be construed as official or reflecting the views of the Navy Department or the naval service at large. The experiments reported herein were conducted according to the principles set forth in the current edition of the ‘Guide for the Care and Use of Laboratory Animals’, Institute of Laboratory Animal Resources, National Research Council. We thank Dr J. Petricciani for continuous support and Ms E. Kirshbaum for typing the manuscript.Google Scholar
  2. 2.
    J.C. Cerottini and K.T. Brunner, Adv. Immun.18, 67 (1974).CrossRefGoogle Scholar
  3. 3.
    In vitro methods in cell-mediated and tumor immunity. B.-R. Bloom and J.R. David (ed.). Academic Press, New York 1976.Google Scholar
  4. 4.
    L.L. Vindelov, Virchows Arch. B. Cell Path.24, 227 (1977).Google Scholar
  5. 5.
    P.D. Noguchi, W.C. Browne, J. Histochem. Cytochem.26, 761 (1978).CrossRefGoogle Scholar
  6. 6.
    A.M. Attallah, in preparation.Google Scholar
  7. 7.
    A.M. Attallah, submitted for publication.Google Scholar

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • A. M. Attallah
    • 1
    • 2
    • 3
  • P. D. Noguchi
    • 1
    • 2
    • 3
  • T. Folks
    • 1
    • 2
    • 3
  • C. T. Noguchi
    • 1
    • 2
    • 3
  1. 1.Division of PathologyBureau of BiologicsBethesda
  2. 2.Naval Medical Research InstituteBethesda
  3. 3.Laboratory of Chemical BiologyNational Institute of Arthritis, Metabolic and Digestive DiseasesBethesdaUSA

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