, Volume 36, Issue 1, pp 119–120 | Cite as

Localization of acid phosphatases in the cell fractions of chick liver

  • F. Panara
  • C. Cirotto
  • I. Arangi
  • L. Barberini


3 molecular forms (P1, P2 and P3) of acid phosphatase (E.C. have been detected in chicken liver homogenate. The different intracellular localization of these molecules has been demonstrated by cellular fractionation and electrophoretic analysis. P1 and P2 phosphatases are both present in the particulate fraction. P3 is present in a pure form in the soluble fraction. The difference between the enzyme molecules present in the particulate fraction and that in the soluble one is confirmed by the different activation-inhibition effect of various ions and substances on the enzymatic activity of subcellular fractions.


Enzyme Enzymatic Activity Fractionation Acid Phosphatase Soluble Fraction 


  1. 1.
    T. Barka, J. Histochem. Cytochem.9, 542 (1961).CrossRefGoogle Scholar
  2. 2.
    J.M. Allen and J. Gockerman, Ann. N.Y. Acad. Sci.121, 616 (1964).CrossRefGoogle Scholar
  3. 3.
    A. Kaneko, T. Ikeda and T. Onóe, Biochim. biophys. Acta222, 218 (1970).CrossRefGoogle Scholar
  4. 4.
    H.D. Campbell, N.P.B. Dudman and B. Zerner, FEBS Lett.31, 123 (1973).CrossRefGoogle Scholar
  5. 5.
    F. Panara and C. Cirotto, IRCS Med. Sci.5, 235 (1977).Google Scholar
  6. 6.
    B.W. Moore and P.U. Angeletti, Ann. N.Y. Acad. Sci.94, 659 (1961).CrossRefGoogle Scholar
  7. 7.
    T. Barka, J. Histochem. Cytochem.9, 564 (1961).CrossRefGoogle Scholar
  8. 8.
    S. Shibko and A.L. Tappel, Biochim. biophys. Acta73, 76 (1963).CrossRefGoogle Scholar
  9. 9.
    M.W. Neil and M.W. Horner, Biochem. J.92, 217 (1964).CrossRefGoogle Scholar
  10. 10.
    M.W. Neil and M.W. Horner, Biochem. J.93, 220 (1964).CrossRefGoogle Scholar
  11. 11.
    K.M. Wang, Biochem. J.115, 191 (1969).CrossRefGoogle Scholar
  12. 12.
    C.F. Strittmatter, Biochim. biophys. Acta284, 183 (1972).CrossRefGoogle Scholar
  13. 13.
    C.F. Strittmatter, Biochim. biophys. Acta284, 196 (1972).CrossRefGoogle Scholar
  14. 14.
    F. Panara, C. Cirotto, I. Arangi and L. Barberini, Experientia34, 983 (1978).CrossRefGoogle Scholar
  15. 15.
    O.H. Lowry, N.J. Rosenbrough, A.L. Farr and R.J. Randall, J. biol. Chem.193, 265 (1951).PubMedGoogle Scholar

Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • F. Panara
    • 1
  • C. Cirotto
    • 1
  • I. Arangi
    • 1
  • L. Barberini
    • 1
  1. 1.Istituto di Istologia ed Embriologia, Facoltà di Scienze MM. FF. NN.University of PerugiaPerugiaItaly

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