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Experientia

, Volume 36, Issue 1, pp 64–65 | Cite as

A study of irrigation fluids for neurosurgery on brain primary cell cultures

  • Elisabeth Hansson
  • B. Vällfors
Specialia

Summary

Primary cell cultures from newborn rat brain hemispheres were exposed to different irrigation fluids used in neurosurgery. The cells died after incubation for 5 min with hydrogen peroxide, and the number of cells was drastically decreased after 10 sec of incubation. They shrank after incubation in Elliott's artificial cerebrospinal fluid for 3 h, but the viability as determined by trypan blue exclusion test was not affected. Physiological sodium chloride, Ringer's solution and the culture medium 199 with Hank's salt had no noticeable effect on the viability or morphology of the cells.

Keywords

Chloride Hydrogen Peroxide Sodium Chloride Cerebrospinal Fluid Trypan Blue 

References

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Copyright information

© Birkhäuser Verlag 1980

Authors and Affiliations

  • Elisabeth Hansson
    • 1
  • B. Vällfors
    • 1
  1. 1.Institute of Neurobiology and Department of NeurosurgeryUniversity of GöteborgGöteborg 33Sweden

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