Pharmaceutisch Weekblad

, Volume 13, Issue 2, pp 78–82 | Cite as

Evaluation of genotoxic risk of handling cytostatic drugs in clinical pharmacy practice

  • Ester P. Guinée
  • Gert H. Beuman
  • Geja Hageman
  • Irene J. Welle
  • Jos C. S. Kleinjans


The genotoxic risk of handling antineoplastic drugs was evaluated in fifteen women preparing chemotherapeutics in the Pharmacy Department of the University Hospital Maastricht. Twenty nurses of the same hospital, who were not exposed to cytostatics, acted as controls. Endogenous exposure to antineoplastic drugs was assessed by determination of urine mutagenicity, as well as by analysis of urinary methotrexate levels. As genotoxicological end-points, sister chromatid exchanges and hypoxanthine guanine phosphoribosyl transferase locus point mutations were studied in peripheral lymphocytes obtained via venous puncture. No differences in urine mutagenic activity, in sister chromatid exchange frequencies and in hypoxanthine guanine phosphoribosyl transferase point mutation frequencies between exposed and non-exposed groups were detected. Higher sister chromatid exchange frequency was observed in smokers as compared to non-smokers.


Antineoplastic agents Hypoxanthine guanine phosphoribosyl transferase Mutagenicity tests Pharmacies, hospital Urine 


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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1991

Authors and Affiliations

  • Ester P. Guinée
    • 1
  • Gert H. Beuman
    • 2
  • Geja Hageman
    • 1
  • Irene J. Welle
    • 1
  • Jos C. S. Kleinjans
    • 1
  1. 1.Department of Biological Health ScienceUniversity of LimburgMD Maastrichtthe Netherlands
  2. 2.Department of Clinical Pharmacy and ToxicologyUniversity Hospital MaastrichtBX Maastrichtthe Netherlands

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