Pharmaceutisch Weekblad

, Volume 4, Issue 4, pp 104–111 | Cite as

The determination of aminoglycoside antibiotics in serum: A comparison of a high performance liquid chromatographic method with a microbiological assay

  • D. M. Barends
  • A. Rutgers
  • B. Van Klingeren
  • A. Hulshoff
Original Articles


A high performance liquid chromatographic (hplc) method for the determination of gentamicin and tobramycin in serum is compared with a microbiological method. The determination of gentamicin by chromatographic methods is complicated by the fact that gentamicin is a mixture of related compounds, giving rise to several chromatographic peaks. Good results were obtained when the sum of the peak heights of the chromatographic peaks corresponding with the gentamicin components are taken as a measure for the gentamicin concentration. Accuracy, precision and selectivity of the methods are discussed.


Public Health Internal Medicine Gentamicin Peak Height Aminoglycoside 
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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1982

Authors and Affiliations

  • D. M. Barends
    • 1
  • A. Rutgers
    • 2
  • B. Van Klingeren
    • 2
  • A. Hulshoff
    • 1
  1. 1.Department of Analytical PharmacyUniversity of Utrecht, Pharmaceutical LaboratoryGH UtrechtThe Netherlands
  2. 2.National Institute of Public Health (riv)BA BilthovenThe Netherlands

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