Advertisement

Pharmaceutisch Weekblad

, Volume 4, Issue 4, pp 104–111 | Cite as

The determination of aminoglycoside antibiotics in serum: A comparison of a high performance liquid chromatographic method with a microbiological assay

  • D. M. Barends
  • A. Rutgers
  • B. Van Klingeren
  • A. Hulshoff
Original Articles

Abstract

A high performance liquid chromatographic (hplc) method for the determination of gentamicin and tobramycin in serum is compared with a microbiological method. The determination of gentamicin by chromatographic methods is complicated by the fact that gentamicin is a mixture of related compounds, giving rise to several chromatographic peaks. Good results were obtained when the sum of the peak heights of the chromatographic peaks corresponding with the gentamicin components are taken as a measure for the gentamicin concentration. Accuracy, precision and selectivity of the methods are discussed.

Keywords

Public Health Internal Medicine Gentamicin Peak Height Aminoglycoside 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Anhalt, J.P., F.D. Sancilio andT. Mccorkle (1978)J. Chromatog. 153, 489–493.Google Scholar
  2. Barends, D.M., J.S.F. Van Der Sandt andA. Hulshoff (1980)J. Chromatog. 182, 201–210.Google Scholar
  3. Barends, D.M., C.L. Zwaan andA. Hulshoff (1981a)J. Chromatog. 222, 316–323;Ibidem (1981b)225, 417–426.Google Scholar
  4. British Pharmacopoeia (1980) The Pharmaceutical Press, London, 208.Google Scholar
  5. Calam, D.H., J.N.T. Gilbert, J.W. Lightbown, J.W. Powell andA.H. Thomas (1978)J. Pharm. Pharmacol. 30, 220–223.Google Scholar
  6. Code of Federal Regulations (1979) 21Cfr 300.50,Us Government Printing Office, Washington, 199, 200.Google Scholar
  7. Davis, R.B., J.E. Thompson andH.L. Pardue (1978)Clin. Chem. 24, 611–620.Google Scholar
  8. Hospes, W., R.J. Boskma andJ.R.B.J. Brouwers (1982)Pharm. Weekbl. Sci. Ed. 4, 32–37.Google Scholar
  9. Klingeren, B. Van, andA. Rutgers (1979)Acta Clin. Belg. 34, 278–287.Google Scholar
  10. Larsen, N-E., andK. Marinelli (1980)J. Chromatog. 221, 182–187.Google Scholar
  11. Maitra, S.K., T.T. Yoshikawa, L.B. Guze andM.C. Schotz (1979)Clin. Chem. 25, 1361–1367.Google Scholar
  12. Mandel, J., andF.J. Linnig (1957)Anal. Chem. 29, 743–749.Google Scholar
  13. Thomas, A.H. (1978)J. Pharm. Pharmacol. 30, 378–379.Google Scholar
  14. Tsuji, K., J.F. Goetz, W. Van Meter andK.A. Gusciora (1979)J. Chromatog. 175, 141–152.Google Scholar
  15. United States Pharmacopoeia (1980) 20th Revision. Mack Publishing Co., Easton, PA, 348, 1332.Google Scholar
  16. Waitz, J.A., andM.J. Weinstein (1968)J. Infect. Diseases 119, 355–360.Google Scholar
  17. Weinstein, M.J., G.H. Wagman, E.M. Oden andJ.A. Marques (1967)J. Bacteriol. 94, 789–790.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1982

Authors and Affiliations

  • D. M. Barends
    • 1
  • A. Rutgers
    • 2
  • B. Van Klingeren
    • 2
  • A. Hulshoff
    • 1
  1. 1.Department of Analytical PharmacyUniversity of Utrecht, Pharmaceutical LaboratoryGH UtrechtThe Netherlands
  2. 2.National Institute of Public Health (riv)BA BilthovenThe Netherlands

Personalised recommendations