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Pharmaceutisch Weekblad

, Volume 5, Issue 4, pp 145–148 | Cite as

Determination of glutathione in biological material by high pressure liquid chromatography

  • A. J. Baars
  • D. D. Breimer
Original Articles

Abstract

A rapid and selective determination of reduced glutathione in biological material is described, based on its conjugation with 1-chloro-2,4-dinitrobenzene. The reaction product has aUv absorption maximum at 340 nm and is analysed by reversed phase high pressure liquid chromatography. Linear calibration graphs were obtained in the concentration range between 50μ M and 2 mM glutathione in standard solutions and in biological material (rat liver and bacterial homogenates). The detection limit is about 2μM glutathione when using 20μl injection samples.

Keywords

Glutathione Reversed Phase Biological Material Reduce Glutathione High Pressure Liquid Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baars. A.J., andD.D. Breimer (1980)Ann. Biol. Clin. 38, 49–56.Google Scholar
  2. Baars, A.J., E.W. van Dongen andD.D. Breimer (1977)Pharm. Weekbl. 112. 1117–1120.Google Scholar
  3. Ellman, G.L. (1959)Arch. Biochem. Biophys. 82, 70–77.PubMedGoogle Scholar
  4. Glazenburg, E.J., I.M.C.Jekel-Halsema. E.Scholtens, A.J.Baars and G.J.Mulder (1983)J. Nutrition. in press.Google Scholar
  5. Habig, W.H., M.J. Pabst andW.B. Jakoby (1974)J. Biol. Chem. 249, 7130–7139.PubMedGoogle Scholar
  6. Hissin, P.J., andR. Hilf (1976)Anal. Biochem. 74, 214–226.PubMedGoogle Scholar
  7. Jakoby. W.B. (1978)Adv. Enzymol. 46. 383–414.PubMedGoogle Scholar
  8. Mohn, G.R., P. DeKnuff and A.J.Baars (1983)Mutation Research Letters, in press.Google Scholar
  9. Orrenius, S., andD.P. Jones (1978) In:Functions of Glutathione in Liver and Kidney (Sies, H., andA. Wendel, Eds.). Springer-Verlag, Berlin, 164–175.Google Scholar
  10. Reeve, J., J. Kuhlenkamp andN. Kaplowitz (1980)J. Chromatogr. 194, 424–428.CrossRefPubMedGoogle Scholar
  11. Reeve, J., J. Kuhlenkamp andN. Kaplowitz (1980)J. Chromatogr. 194, 424–428.CrossRefPubMedGoogle Scholar
  12. VanBladeren, P.J., (1981)The Dual Role of Glutathione Conjugation in the Biotransformation of Xenobiotics. Ph.D. thesis, University of Leiden.Google Scholar
  13. van Bladeren, P.J., D.D. Breimer, G.M.T. Rotteveel-Smus, P. de Knijff, G.R. Mohn, B. van Meeteren-Wälchli, W. Buijs andA. van der Gen (1981)Carcinogenesis 2, 499–505.PubMedGoogle Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1983

Authors and Affiliations

  • A. J. Baars
    • 1
  • D. D. Breimer
    • 1
  1. 1.Department of Pharmacology, Subfaculty of PharmacyUniversity of Leiden, Sylvius LaboratoriesRA LeidenThe Netherlands

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