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Pharmaceutisch Weekblad

, Volume 6, Issue 1, pp 7–10 | Cite as

The Shimadzu CS 920 densitometer: illumination of thin layer chromatograms with a parallel light beam and its effects on quantification

  • F. A. Huf
Original Articles

Abstract

The Shimadzu CS 920 has shown to be an easy to handle flying-spot densitometer that enables rapid quantification of thin layer chromatograms. Linear calibration curves with a relative standard deviation of 3 to 4% in the slope can be obtained for Sudan Yellow on Merck H60 plates. However, contrary to the statements in the instrument manual, the ‘linearizer’ performance is not in agreement with the Kubelka and Munk theory. Consequently, the CS 920 can only be used after empirically determined adjustment of the ‘linearizer’. Furthermore, errors in analysis can increase up to 30% by using TLC-plates with fluctuating layer thickness.

Keywords

Public Health Standard Deviation Internal Medicine Calibration Curve Thin Layer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Kubelka P, Munk F. Ein Beitrag zur Optik der Farbanstriche. Z Techn Physik 1931:12;593–603.Google Scholar
  2. 2.
    Huf FA. Application of the Kubelka and Munk theory to densitometry: simultaneous transmittance and reflectance measurements. Anal Chim Acta 1977:70;143–50.Google Scholar
  3. 3.
    Huf FA, De Jong HJ, Schute JB. Application of the Kubelka and Munk theory to densitometry: transmittance measurements. Anal Chim Acta 1976:85;341–9.Google Scholar
  4. 4.
    Huf FA. Evaluation of the Kubelka and Munk theory of light absorption in scattering media for quantitative thin layer chromatography. Leiden: University of Leiden, 1978. 148 p.p. Thesis.Google Scholar
  5. 5.
    Ebel S, Geitz E, Hocke J. In: Kaiser RE, ed. Instrumental HPTLC. Heidelberg: Hütig Verlag, 1980:55–80.Google Scholar
  6. 6.
    Huf FA. Advances in Steroid Analysis. In: Görög S, ed. Symposium on the Analysis of Steroids. Amsterdam: Elsevier Publishing Company, 1982.Google Scholar
  7. 7.
    Anonymus. Shimadzu manual. Kyoto: Shimadzu Corporation, 1980.Google Scholar
  8. 8.
    Goldman J, Goodall R. Quantitative analysis of thin layer chromatograms; application of an optical theory to ‘in situ’ analysis. J Chromatogr 1969;40:345–58.Google Scholar
  9. 9.
    Yamamoto H. In: Kaiser RE, ed. Instrumental HPTLC. Heidelberg: Hüttig Verlag, 1980:367–84.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1984

Authors and Affiliations

  • F. A. Huf
    • 1
  1. 1.Department of Analytical Chemistry and Pharmaceutical AnalysisUniversity of Leiden, Gorlaeus LaboratoriesRA LeidenThe Netherlands

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