Pharmaceutisch Weekblad

, Volume 11, Issue 5, pp 169–174 | Cite as

Analysis of water-soluble vitamins in total parenteral nutrition solution by high pressure liquid chromatography

  • A. van der Horst
  • H. J. M. Martens
  • P. N. F. C. de Goede
Original Articles


A stability-indicating high pressure liquid chromatographic method was developed to determine the stability of water-soluble vitamins in total parenteral nutrition mixtures. Folic acid and thiamine were determined by direct injection and precolumn enrichment, followed by UV detection. Nicotinamide and pyridoxine were determined simultaneously without sample pretreatment by UV detection of nicotinamide and fluorescence detection of pyridoxine. Riboflavin 5′-phosphate was also determined without elaborate sample handling and by fluorescence detection. Ascorbic acid was determined as the sum of ascorbic acid and dehydroascorbic acid. After derivatization to a quinoxaline the latter substance was determined by direct injection and fluorescence detection.


Ascorbic acid Chromatography, high pressure liquid Drug stability Folic acid Nicotinamide Parenteral nutrition Pyridoxine Quantitative analysis Riboflavin 5′-phosphate Thiamine 


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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1989

Authors and Affiliations

  • A. van der Horst
    • 1
  • H. J. M. Martens
    • 1
  • P. N. F. C. de Goede
    • 1
  1. 1.Laboratory for Drug Analysis and Toxicology, Department of PharmacyFree University HospitalHV Amsterdamthe Netherlands

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