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Pharmaceutisch Weekblad

, Volume 4, Issue 6, pp 176–182 | Cite as

Enzymatic O-methylation of catechols and catecholamines

  • M. T. I. W. Schüsler-Van Hees
  • G. M. J. Beijersbergen Van Henegouwen
Original Articles

Abstract

The reactivity of a number of catechols and catecholamines with regard to the enzymaticO-methylation by catechol-O-methyltransferase (comt) was studied. The reaction was carried out in a vial at a temperature of 37°C, the vial contained a certain concentration of one catechol(amine), catechol-O-methyltransferase (the enzyme),S-adenosylmethionine (the methyldonor), MgCl2 (a cofactor) and buffer pH=7.85. After certain time intervals samples were taken, the reaction was stopped in acid. The catechol(amine) concentration decrease and the product concentration increase were determined by injecting the samples directly into anhplc connected with a fluorimeter, giving the opportunity of estimating the mass balance. Vmax, Km, C3/C4 ratio (=ratio of 3-O- and 4-O-methylated product formed) and the reaction rate at low substrate (catecholamine) concentration (=Vmax.[S]/ Km) — which appears to be related to log P — are given.

It is conjectured that V at low substrate concentration is especially determined by the polarity of the catechol(amine) while Vmax is primarily determined by other physico-chemical properties like steric conformation (l-dopavsdl-dopa;l-adrenalinevsdl-adrenaline).

Keywords

Public Health Internal Medicine MgCl2 Catecholamine Mass Balance 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1982

Authors and Affiliations

  • M. T. I. W. Schüsler-Van Hees
    • 1
  • G. M. J. Beijersbergen Van Henegouwen
    • 1
  1. 1.Subfaculty of PharmacyState University of Leiden. Gorlaeus LaboratoriesRA LeidenThe Netherlands

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