Pharmaceutisch Weekblad

, Volume 4, Issue 6, pp 172–175 | Cite as

Determination of amygdalin and its major metabolite prunasin in plasma and urine by high pressure liquid chromatography

  • A. G. Rauws
  • L. G. Gramberg
  • M. Olling
Original Articles


A method is described for the determination of amygdalin and prunasin in plasma ultrafiltrate and urine. Both compounds are separated by high pressure liquid chromatography on a reversed phase column and subsequently detected at 215 nm. The identity of an amygdalin metabolite with prunasin was confirmed by mass spectrometry.


Public Health Mass Spectrometry Internal Medicine Liquid Chromatography High Pressure 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Ames, M.M., T.P. Moyer, J.S. Kovach, C.G. Moertel andJ.R. Rubin (1981)Cancer Chemotherap. Pharmacol. 6, 51–57.Google Scholar
  2. Berzelius, J.J. (1838)Lehrbuch der Chemie, 3. Auflage, BandVii. Arnoldische Buchhandlung, Dresden-Leipzig, 512.Google Scholar
  3. Cairns, T., J.E. Froberg, S. Gonzales, W.S. Langham andJ.J. Stamp (1978)Anal. Chem. 50, 317–322.Google Scholar
  4. Carter, J.H., M.A. Mclafferty andP. Goldman (1980)Biochem. Pharmacol. 29, 301–304.Google Scholar
  5. Editorial (1977)Vet. Hum. Toxicol. 21, 112–114.Google Scholar
  6. Flora, K.P., J.C. Cradock andM.M. Ames (1978)Res. Comm. Chem. Pathol. Pharmacol. 20, 367–378.Google Scholar
  7. Haworth, W.N., andB. Wylam (1923)J. Chem. Soc. 123, 3120–3125.Google Scholar
  8. Khandekar, J.D., andH. Edelman (1979).J. Am. Med. Assoc. 242, 169–171.Google Scholar
  9. Moertel, C.G., M. Ames, J.S. Kovach, R.P. Moyer, J.R. Rubin andJ.H. Tinker (1981)J. Am. Med. Assoc. 245, 591–594.Google Scholar
  10. Nikolelis, D.P., andT.P. Hadjiioannou (1979)Anal. Letters B12, 1169–1178.Google Scholar
  11. Rauws, A.G., M. Olling andA. Timmerman (1982)Arch. Toxicol. 49, 311–319.Google Scholar
  12. Schmidt, E.S., G.W. Newton, S.M. Sanders, J.P. Lewis andE.E. Conn (1978)J. Am. Med. Assoc. 239, 943–947.Google Scholar
  13. Stobaugh, J.F., L.A. Sternson andA.J. Repta (1978)Anal. Letters B11, 753–764.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1982

Authors and Affiliations

  • A. G. Rauws
    • 1
  • L. G. Gramberg
    • 1
  • M. Olling
    • 1
  1. 1.National Institute of Public HealthBA BilthovenThe Netherlands

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