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Pharmaceutisch Weekblad

, Volume 9, Issue 5, pp 261–264 | Cite as

Determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids by reversed-phase high pressure liquid chromatography

  • E. H. H. Wiltink
  • P. Stekkinger
  • J. A. C. Brakenhoff
  • S. A. Danner
Original Articles

Abstract

In this article a simple method for the determination of 9-(1,3-dihydroxy-2-propoxymethyl)guanine (DHPG) in biological fluids is presented, based on reversed-phase ion-pair HPLC with UV detection at 254 nm. No complicated extraction procedure is needed. The stationary phase consists of a reversed-phase C18 stainless steel column and the mobile phase of 0.005M sodium acetate and 0.0025M pentanesulfonic acid sodium salt (PIC-B5) as an ion-pair reagent in water (pH 6.5). DHPG is a new antiviral drug in research of the treatment of cytomegalovirus infections. As a pilot study serum and urine samples of a few patients were investigated to gain an impression of the therapeutic range of DHPG. The method is suitable for routine assay of DHPG.

Key words

Chromatography, high pressure liquid Detection, ultraviolet 9-(1,3-Dihydroxy-2-propoxymethyl)guanine Serum Urine 

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Copyright information

© Bohn, Scheltema & Holkema 1987

Authors and Affiliations

  • E. H. H. Wiltink
    • 1
  • P. Stekkinger
    • 1
  • J. A. C. Brakenhoff
    • 2
  • S. A. Danner
    • 2
  1. 1.Department of PharmacyAcademic Medical CentreAZ Amsterdamthe Netherlands
  2. 2.Department of Internal MedicineAcademic Medical CentreAZ Amsterdamthe Netherlands

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