Pharmacy World and Science

, Volume 17, Issue 2, pp 54–57 | Cite as

Colorimetric determination of some amino acids containing a sulfur group

  • A. M. El-Brashy
  • S. M. Al-Ghannam
Practice/Case Reports


A simple and rapid colorimetric method for the determination of some amino acids containing a sulfur group is described. The studied compounds are cysteine, cystine and methionine. The method is based on the formation of ferroin, from the reaction of the studied drugs with a mixture of iron(III) and 1,10-phenanthroline and measurement of the absorbance at 512 nm. The procedure has been successfully applied to the assay of the pharmaceutical preparations of the studied drugs after thinlayer Chromatographic separation and the results of the studied compounds compare favourably with the official methods.


Amino acids, sulfur Chromatography, thin layer Colorimetry Iron Phenanthrolines 


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  1. 1.
    Mac Donald JL, Krueger MW, Keller JH. Oxidation and hydrolysis determination of sulphur amino acids in food and feed ingredients: collaborative study. J Assoc Off Anal Chem 1985;68:826–9.Google Scholar
  2. 2.
    Mathur NK, Narang CK. Determination of organic compounds withN-bromosuccinimide and allied reagents. London: Academic Press, 1974:88–114.Google Scholar
  3. 3.
    Shukla IC, Chauhan RS, Prakash R. A new titrimetric method for the determination of thioureas and thioamino acids. Proc Natl Acad Sci USA 1981;51(2):149–52.Google Scholar
  4. 4.
    Mahadevappa DS, Rangappa KS, Gowda NM. Oxidimetric determination of methionine and its metal complexes with chloramine-B and dichloramine-B. Microchem J 1983;28(2):235–49.Google Scholar
  5. 5.
    Jameson RF, Linert W, Tschinkowitz A. Anaerobic oxidation of cystein to cystine by iron(III). Part 2. The reaction in basic solution. J Chem Soc Dalton Trans 1988;12:2109.Google Scholar
  6. 6.
    Welcher FJ. Standard methods of chemical analysis. New York: Robert E. Kreiger, 1975:920–49.Google Scholar
  7. 7.
    Fabre H, Blanchin MD. Application of spectrophotometry of thin-layer chromatograms to analysis for amino-acids in formulations for veterinary use. Pharm Acta Helv 1983;58(2):52–4.Google Scholar
  8. 8.
    Jentsch J. The use of a new silica gel separation system in thin-layer chromatography. Amino Acids 1991;1(2):279–81.Google Scholar
  9. 9.
    Carta G, Saunders MS, Mawengkang F. Studies on the diffusion of amino acids in ion-exchange resins. Int Conf Fund Adsorpt 1989:181–90.Google Scholar
  10. 10.
    United States Pharmacopeia XXII/National Formulary XVII. Rockville: United States Pharmacopeial Convention, 1990.Google Scholar
  11. 11.
    Huang Y, Lin L, Chee J. Simultaneous determination of cystine and lysine by oscillopolarography. Fenxi Ceshi Tongbao 1992;11(1):72–4.Google Scholar
  12. 12.
    Katacka H, Ohishi K, Sumida Y, Ohmori M, Makita M. Gaschromatographic assay for cysteine sulphinate decarboxylase [sulphinoalanine decarboxylase] activity in animal tissues. J Chromatogr Biomed Appl 1987;64:135–40.Google Scholar
  13. 13.
    Moodie IM, Hough BJ, Labadorios D. Determination of amino acids in urine by gas chromatography. J High Res Chromatogr 1989;12:437–41.Google Scholar
  14. 14.
    Brueckner H, Langer M, Godel H. LC-determination of Dand L-amino acids in peptide hydrolyzates by pre-column derivatization with a chiral reagent. Tech Protein Chem 1991;3:243–7.Google Scholar
  15. 15.
    Jenke DR, Brown DS. Determination of cysteine in pharmaceuticals via liquid chromatography with post-column derivatization. Anal Chem 1987;59(11):1509–12.Google Scholar
  16. 16.
    Dou L, Krull IS. Determination of aromatic and sulphur containing amino acids, peptides and proteins using high performance liquid chromatography with photolytic electrochemical detection. Anal Chem 1990;62:2599–606.Google Scholar
  17. 17.
    Canevari L, Vieira R, Aldegunde M, Dagani F. High-performance liquid Chromatographic separation with electrochemical detection of amino acids focusing on neurochemical application. Anal Biochem 1992;205(1):137–42.Google Scholar
  18. 18.
    Burguera JL, Burguera M, De la Guardia M, Salvador A. Pyrolysis-flow injection analysis-spectrophotometric determination of amino acids in aqueous solutions. Anal Chim Acta 1992;261(1–2):23–7.Google Scholar
  19. 19.
    Hui BS, Huber CO. Amperometric detection of amines and amino acids in flow injection systems with a nickel oxide electrode. Anal Chim Acta 1982;134:211–8.Google Scholar
  20. 20.
    Roth HJ, Eger K, Troschutz R. Pharmaceutical chemistry. Vol. 2. Drug analysis. New York: Ellis Horwood, 1991:204–39.Google Scholar
  21. 21.
    Prakash A, Singh AP, Shukla IC. Spectrophotometric method for the determination of some thioamino acids and thioureas in pure and pharmaceutical preparations. J Indian Chem Soc 1987;64(4):262–4.Google Scholar
  22. 22.
    Watanabe Y, Imai K. Determination of amino acids, imino acids and thiols by post column derivatization with 4-fluoro-7-nitrobenzo-2,1,3-oxadiazole. Anal Chem 1983;55:1786–91.Google Scholar
  23. 23.
    Stahl E. Thin layer chromatography. Berlin: Springer-Verlag, 1969:60.Google Scholar
  24. 24.
    Morris CN, Rolph JE. Introduction to data analysis and statistical inference. London: Prentice-Hall, 1981.Google Scholar

Copyright information

© Royal Dutch Association for Advancement of Pharmacy 1995

Authors and Affiliations

  • A. M. El-Brashy
    • 1
  • S. M. Al-Ghannam
    • 1
  1. 1.Department of ChemistryGirls College of ScienceDammam 838Kingdom of Saudi Arabia

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