Summary
This report describes procedures for the isolation and maintenance of monolayer culture of adult rat liver hepatic parenchymal cells. Isolation of the cells is accomplished using perfusion in situ with a calcium-free buffer followed by buffered collagenase. Gravity sedimentation and selective media are used to limit the contribution of nonparenchymal cells in the cultures.
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McGowan, J.A., Bucher, N.L.R. The isolation of adult rat liver parenchymal cells. Journal of Tissue Culture Methods 9, 49–52 (1985). https://doi.org/10.1007/BF01797774
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DOI: https://doi.org/10.1007/BF01797774