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Documenta Ophthalmologica

, Volume 26, Issue 1, pp 185–191 | Cite as

Cholinesterase isoenzymes in rabbit ocular tissue homogenates

  • Moses Furman
  • Moshe Lazar
  • Irving H. Leopold
Article

Summary

Proteins of rabbit ocular tissue homogenates were separated by discelectrophoresis and analyzed for cholinesterase. By the use of specific substrates and selective inhibitors it is shown that true cholinesterase is largely present in ocular tissue with a small amount of nsChE also present. The number of isoenzymes (bands) varies with a given tissue.

Keywords

Cholinesterase Ocular Tissue Acetylthiocholine Thiocholine Serum Cholinesterase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Résumé

A partir d'homogénats de tissus oculaires du lapin on a séparé les protéines pas électrophorèse sur disque et l'on y a recherché la cholinestérase. En utilisant des substrats spécifiques et des inhibiteurs sélectifs on a pu démontrer que les tissus oculaires renferment surtout de la vraie (acétyl) cholinestérase. Certains d'entre eux contiennent aussi de petites quantités de pseudocholinestérase (non spécifique). Le nombre des isoenzymes varie d'un tissu à l'autre.

Zusammenfassung

Im Homogenat von Augengeweben des Kaninchens wurden die Proteine durch Diskelektrophorese getrennt und auf Cholinesteraseanwesenheit untersucht. Mit Hilfe von spezifischen Substraten und selektiven Hemmstoffen konnte hauptsächlich wahre (Acetyl) Cholinesterase nachgewiesen werden, jedoch ist auch eine geringe Menge von Pseudocholinesterase (unspezifisch) in einzelnen Geweben vorhanden. Die Zahl ihrer Isoenzyme ist verschieden.

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Copyright information

© Dr. W. Junk N.V. 1969

Authors and Affiliations

  • Moses Furman
    • 1
  • Moshe Lazar
    • 1
  • Irving H. Leopold
    • 1
  1. 1.Department of OphthalmologyMount Sinai School of Medicine of the City UniversityNew YorkUSA

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