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Intraspecific 5S rRNA gene variation in flax,Linum usitatissimum (Linaceae)

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Abstract

A relatively large proportion of the flax genome (~ 3%) is comprised of 5S rRNA genes. This study focuses on the intraspecific sequence variation among five distinct groups of 5S rRNA genes. The results indicate that group 1 and 2 5S rRNA genes most closely resemble other angiosperm 5S genes, while groups 3–5 are highly divergent. Sequence variation is higher in the spacer region compared to the transcribed region for all pairwise comparisons. The large degree of sequence variation observed in this study is discussed with respect to genome organization and proposed models for repetitive sequence maintenance.

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References

  1. Appels, R., Gerlach, W. L., Dennis, E. S., Swift, H., Peacock, W. J., 1980: Molecular and chromosomal organization of DNA sequences coding for the ribosomal RNAs in cereals. — Chromosoma78: 293–311.

  2. Blanco, J., Millestein, L., Razik, M. A., Dilworth, S., Cote, C., Gottesfeld, J., 1989: Two TFIIIA activities regulate expression of theXenopus 5S RNA gene families. — Genes & Development3: 1602–1612.

  3. Brown, D. D., Wensink, P. C., Jorda, E., 1972: A comparison ofXenopus laevis andXenopus mulleri: the evolution of tandem genes. — J. Mol. Biol.89: 703–718.

  4. Cullis, C. A., 1977: Molecular aspects of the environmental induction of heritable changes in flax. — Heredity38: 129–154.

  5. Dover, G., 1982: Molecular drive: a cohesive mode of species evolution. — Nature299: 111–117.

  6. Ellis, T. H. N., Lee, D., Thomas, C. M., Simpson, P. R., Cleary, W. G., Newman, M. A., Burcham, K. W. G., 1988: 5S rRNA genes inPisum: sequence, long range and chromosomal organization. — Mol. Gen. Genet.214: 333–342.

  7. Erdmann, V. A., Wolters, J., 1986: Collection of published 5S, 5.8S, and 4.5S ribosomal RNA sequences. — Nucl. Acids Res. Suppl.14: r1-r35.

  8. Fedoroff, N. V., 1979: On spacers. — Cell16: 697–710.

  9. , 1978: The nucleotide sequence of oocyte 5S DNA inXenopus laevis. I. The AT rich spacer. — Cell73: 701–716.

  10. Goldsbrough, P. B., Ellis, T. H. N., Cullis, C. A., 1981: Organization of the 5S RNA genes in flax. — Nucl. Acids Res.9: 5895–5904.

  11. , 1982: Sequence variation and methylation of the flax 5S RNA genes. — Nucl. Acids Res.15: 4501–4514.

  12. Gorman, S. W., Teasdale, R., Cullis, C. A., 1992: Structure and Organization of the 5S rRNA genes (5S DNA) inPinus radiata (Pinaceae). Pl. Syst. Evol.183: 223–234.

  13. Gottlob-McHugh, S. G., Levesque, M., MacKenzie, K., Olson, M., Yarosh, O., Johnson, D. A., 1990: Organization of the 5S rRNA genes in the soybeanGlycine max (L.)Merrill and conservation of the 5S rDNA repeat structure in higher plants. — Genome33: 486–494.

  14. Hariharan, N., Reddy, P. S., Padayatty, J. D., 1987: 5S rRNA genes in rice embryos. — Pl. Mol. Biol.9: 443–451.

  15. Hemleben, V., Werts, W., 1988: Sequence organization and putative regulatory elements in the 5S rRNA genes of two higher plants (Vigna radiata andMatthiola incana). — Gene62: 165–169.

  16. Knälmann, M., Burger, E., 1988: Chromosomal localization of 5S rRNA genes inVicia narbonensis. — Caryologia39: 217–226.

  17. Koller, B. H., Smithies, O., 1990: Inactivation of the β2 microglobulin locus in mouse embryonic stem cells by homologous recombination. — Proc. Natl. Acad. Sci. USA86: 8932–8935.

  18. Korn, L. J., 1982: Transcription ofXenopus 5S ribosomal RNA genes. — Nature295: 101–105.

  19. Little, R. D., Braaten, D. C., 1989: Genomic organization of human 5S rDNA and sequence of one tandem repeat. — Genomics4: 376–383.

  20. Long, E. O., Dawid, I. B., 1980: Repeated genes in eukaryotes. — Ann. Rev. Biochem.49: 727–764.

  21. Maizels, N., 1976:Dictyostelium 17S, 25S, and 5S rDNAs lie within a 38,000 base pair repeated unit. — Cell9: 431–438.

  22. Mascia, P. N., Rubenstein, I., Philliph, R. I., Wang, A. S., Xiang, L. Z., 1989: Localization of the 5S RNA genes and evidence for diversity in the 5S rDNA region of maize. — Gene15: 7–20.

  23. Nagylaky, T., Petes, T. D., 1982: Intra chromosomal gene conversion and the maintenance of sequence homogeneity among repeated genes. — Genetics100: 315–337.

  24. Nazar, R. N., Van Ryk, D. I., Lee, Y., Guyer, C. D., 1991: Use of mutant RNAs in studies on yeast 5S rRNA structure and function. — Biochem. Cell. Biol.69: 217–222.

  25. Ohta, T., 1976: Simple model for treating evolution of multigene families. — Nature263: 74–75.

  26. Pardue, M. L., Brown, D. D., Birnstiel, M. L., 1973: Location of the genes for 5S ribosomal RNA inXenopus laevis. — Chromosoma42: 191–203.

  27. Peterson, R. C., Doering, J. L., Brown, D. D., 1980: Characterization of twoXenopus somatic 5S DNAs and one minor oocyte specific 5S DNA. — Cell20: 131–141.

  28. Reddy, P., Appels, R., 1989: A second locus for the 5S multigene family inSecale L.: sequence divergence in two lineages of the family. — Genome32: 456–467.

  29. Romby, P., Baudin, F., Brunel, C., Leal de Stevenson, I., Westhof, E., Romaniuk, P. J., Ehresmann, C., Ehresmann, B., 1990: Ribosomal 5S RNA fromXenopus laevis oocytes: conformation and interaction with transcription factor IIIA. — Biochimie72: 437–452.

  30. Romaniuk, P. J., Leal de Stevenson, I., Quimin You, 1989: The specificity of the RNA binding activity ofXenopus transcription factor IIIA. — InCech, T., (Ed.): Molecular biology of RNA, pp. 123–132. — Los Angeles: UCLA press.

  31. Rubin, G. M., Suston, J. E., 1973: Physical linkage of the 5S cistrons to the 18S and 28S ribosomal RNA cistrons inSaccharomyces cerevisiae. — J. Mol. Biol.76: 521–530.

  32. Sambrook, J., Fritsch, E. F., Maniatis, T., 1989: Molecular cloning. A laboratory manual, 2nd edn. — Cold Spring Harbor, New York: Cold Spring Harbor Laboratory.

  33. Scoles, G. J., Gill, B. S., Xin, Z.-Y., Clarke, B. C., McIntyre, C. L., Chapman, C., Appels, R., 1988: Frequent duplication and deletion events in the 5S RNA genes and the associated spacer regions of theTriticeae. — Pl. Syst. Evol.160: 105–122.

  34. Schneeberger, R. G., 1992: Characterization of DNA polymorphisms associated with environmentally induced heritable changes in flax. — Ph. D. Thesis. Case Western Reserve University, Cleveland, OH 44106.

  35. , 1991: Specific DNA alterations associated with the environmental induction of heritable changes in flax. — Genetics128: 619–630.

  36. , 1989: Chromosomal and molecular analysis of 5S RNA gene organization in the flax,Linum usitatissimum. — Gene83: 75–84.

  37. Selker, E. U., Stevens, J. N., Metzenberg, R. L., 1985: Heterogeneity of 5S RNA in fungal ribosomes. — Science227: 1340–1343.

  38. , 1981: Dispersed 5S RNA genes inN. crassa: structure, expression and evolution. — Cell24: 819–828.

  39. Smith, G. P., 1976: Evolution of repeated DNA sequences by unequal crossover. — Science191: 528–535.

  40. Tabor, S., Richardson, C. C., 1987: DNA sequence analysis with a modified bacteriophage T 7 DNA polymerase. — Proc. Natl. Acad. Sci. USA84: 4767–4771.

  41. Tyler, B. M., 1987: Transcription ofNeurospora crassa 5S rRNA genes requires a TATA box and three internal elements. — J. Mol. Biol.196: 801–811.

  42. Vandenberghe, A., Chen, E., Dams, M.-W., de Baere, R., de Roeck, R., Huysmans, E., de Wachter, R., 1984: The corrected nucleotide sequences of 5S RNAs from six angiosperms. — FEBs Letters171: 17–23.

  43. Wolffe, A. P., Brown, D. D., 1988: Developmental regulation of two 5S ribosomal RNA genes. — Science241: 1626–1632.

  44. Wolters, J., Erdmann, V. A., 1986: Cladistic analysis of 5S rRNA and 16S rRNA secondary and primary structure: the evolution of eukaryotes and their relation to Archaebacteria. — J. Mol. Evol.24: 152–166.

  45. Zimmer, E. A., Martin, S. L., Beverly, S. M., Kan, Y. W., Wilson, A. C., 1980: Rapid duplication and loss of genes coding for the chains of hemoglobin. — Proc. Natl. Acad. Sci. USA77: 2158–2162.

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Schneeberger, R.G., Cullis, C.A. Intraspecific 5S rRNA gene variation in flax,Linum usitatissimum (Linaceae). Pl Syst Evol 183, 265–280 (1992). https://doi.org/10.1007/BF00940808

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Key words

  • Linaceae
  • Linum usitatissimum
  • 5S rDNA sequence
  • sequence comparisons
  • repetitive DNA
  • concerted evolution