Utilizing the γ-replacement reaction ofStreptomyces cystathionine γ-lyase (EC 22.214.171.124.), an efficient production method forl-cystathionine has been established. Under optimal conditions, 50 mMl-cystathionine was synthesized from 50 mMO-succinyl-l-homoserine and 50 mMl-cysteine, added in four stages to the reaction mixture, with a substrate conversion rate of 100%. This productivity (11 gl-1 of reaction mixture) is about 3.5 times higher than that withl-homoserine andl-cysteine as substrates.
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Recipient of a JSPS Fellowship for Japanese Junior Scientists
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Kanzaki, H., Nagasawa, T. & Yamada, H. Highly efficient production ofl-cystathionine fromO-succinyl-l-homoserine andl-cysteine byStreptomyces cystathionine γ-lyase. Appl Microbiol Biotechnol 25, 97–100 (1986). https://doi.org/10.1007/BF00938931
- Optimal Condition
- Conversion Rate
- Efficient Production
- Production Method