Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Highly efficient production ofl-cystathionine fromO-succinyl-l-homoserine andl-cysteine byStreptomyces cystathionine γ-lyase

  • 18 Accesses

Summary

Utilizing the γ-replacement reaction ofStreptomyces cystathionine γ-lyase (EC 4.4.1.1.), an efficient production method forl-cystathionine has been established. Under optimal conditions, 50 mMl-cystathionine was synthesized from 50 mMO-succinyl-l-homoserine and 50 mMl-cysteine, added in four stages to the reaction mixture, with a substrate conversion rate of 100%. This productivity (11 gl-1 of reaction mixture) is about 3.5 times higher than that withl-homoserine andl-cysteine as substrates.

This is a preview of subscription content, log in to check access.

References

  1. Brenton DP, Cusworth DC, Gaull GE (1965) Homocystinuria — Biochemical studies of tissues including a comparison with cystathioninuria. Pediatrics 35:50–56

  2. Flavin M, Delavier-Klutchko C, Slaughter C (1963) Succinic ester and amide of homoserine: some spontaneous and enzymatic reactions. Science 143:50–52

  3. Gerritsen T, Waisman HA (1964) Homocystinuria: Absence of cystathionine in the brain. Science 145:588

  4. Hendrickson HR, Giovanelli J, Mudd SH (1970) The synthesis ofO-acylamino acids. J Org Chem 35:4270–4273

  5. Kanzaki H, Kobayashi M, Nagasawa T, Yamada H (1986) Syntheses ofS-substitutedl-homocysteine derivatives by cystathionine γ-lyase ofStreptomyces phaeochromogenes. Agric Biol Chem 50:391–397

  6. Kase H, Nakayama K, Kinoshita S (1970) Production ofO-succinyl-l-homoserine by auxotrophic mutants ofAerobacter aerogenes. Agric Biol Chem 34:274–281

  7. Nagai S, Flavin M (1967) Acetylhomoserine — an intermediate in the fungal biosynthesis of methionine. J Biol Chem 242:3884–3895

  8. Nagai S, Flavin M (1971) Synthesis ofO-acetylhomoserine. Methods Enzymol 17B:423–424

  9. Nagasawa T, Kansaki H, Yamada H (1984) Cystathionine γ-lyase ofStreptomyces phaeochromogenes — the occurrence of cystathionine γ-lyase in filamentous bacteria and its purification and characterization. J Biol Chem 259:10393–10403

  10. Okumura N, Otsuki S, Kameyama A (1960) Studies on free amino acids in human brain. J Biochem 47:315–320

  11. Painter EP (1947) A synthesis of selenium analogs ofdl-methionine anddl-homocysteine. J Amer Chem Soc 69:232–234

  12. Rowbury RJ, Woods DD (1964)O-Succinylhomoserine as an intermediate in the synthesis of cystathionine byEscherichia coli. J Gen Microbiol 36:341–358

  13. Tallan HH, Moore S, Stein WH (1958)l-Cystathionine in human brain. J Biol Chem 230:707–716

  14. Werman R, Davidoff RA, Aprison MH (1966) The inhibitory action of cystathionine. Life Sci 5:1431–1440

  15. Wiebers JL, Garner HR (1967) Acyl derivatives of homoserine as substrates for homocysteine synthesis inNeurospora crassa, yeast andEscherichia coli. J Biol Chem 242:5644–5649

  16. Yamada H, Kanzaki H, Nagasawa T (1984) Synthesis ofl-cystathionine by the γ-replacement reaction of cystathionine γ-lyase fromStreptomyces phaeochromogenes. J Biotechnol 1:205–217

Download references

Author information

Correspondence to Hiroshi Kanzaki.

Additional information

Recipient of a JSPS Fellowship for Japanese Junior Scientists

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Kanzaki, H., Nagasawa, T. & Yamada, H. Highly efficient production ofl-cystathionine fromO-succinyl-l-homoserine andl-cysteine byStreptomyces cystathionine γ-lyase. Appl Microbiol Biotechnol 25, 97–100 (1986). https://doi.org/10.1007/BF00938931

Download citation

Keywords

  • Optimal Condition
  • Conversion Rate
  • Efficient Production
  • Production Method
  • Cystathionine