Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Sensorized on line determinations of molecular hydrogen inEscherichia coli fermentations

  • 14 Accesses


The production of molecular hydrogen was measured in the effluent gas of 7 fermentations. The aim of this primary investigation was to study the use of a H2-sensitive metal-oxide-semiconductor structure in physiological studies ofEscherichia coli. In order to yield more information, we measured metabolic heat with a flow microcalorimeter in parallel with the determinations of molecular hydrogen. The physiological parameters of intrest were medial effects, HCOOH-additions, pH and partial oxygen pressure.

This is a preview of subscription content, log in to check access.


  1. Cleland N, Hörnsten EG, Elwing H, Enfors S-O, Lundström I (1984) Measurement of hydrogen evolution by oxygen-limitedEscherichia coli by means of a hydrogen sensitive PD-MOS sensor. Appl Microbiol Biotechnol 20:268–270

  2. Danielsson B, Mattiasson B, Mosbach K (1981) Enzyme thermistor devices and their analytical applications. Appl Biochem Bioeng 3:97–143

  3. Gest H (1954) Oxidation and evolution of molecular hydrogen by microorganisms. Bact Rev 18:43–73

  4. Hörnsten EG, Elwing H, Kihlström E, Lundström I (1985a) Sensorized determination of molecular hydrogen in investigations of the susceptibility of Enterobacteriaceae to ampicillin. J Antimicrob Chemother 15:695–700

  5. Hörnsten EG, Danielsson B, Nilsson L, Elwing H, Lundström I (1985b) Physiological Studies of anEscherichia coli strain under ampicillin stress, anaerobic conditions and spheroplast formation. J Antimicrob Chemother (in press)

  6. Kondratieva EN, Gogotov IN (1983) Production of molecular hydrogen in microorganisms. Advances in Biochemical Engineering/Biotechnology 28:139–191

  7. Krab K, Oltmann LF, Stouthammer A (1982) Reduction of oxygen by hydrogen in cells of anaerobically grown Proteus mirabilis. Biochem Biophys Acta 682:59–66

  8. Lundström I (1981) Hydrogen sensitive MOS-structures Part 1. Principles and Applications. Sensors Actuators 1:403–426

  9. Schlegel HG, Schneider K (1978). In: Goltze E (ed) Hydrogenases; Their Catalytic Activity, Structure and Function. Göttingen

  10. Winquist F, Spetz A, Lundström I (1984) Determination of ammonia in air and aqueous samples with a gas-sensitive semiconductor capacitor. Analytica Chimica Acta 164:127–130

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Hörnsten, E.G., Danielsson, B., Elwing, H. et al. Sensorized on line determinations of molecular hydrogen inEscherichia coli fermentations. Appl Microbiol Biotechnol 24, 117–121 (1986). https://doi.org/10.1007/BF00938781

Download citation


  • Oxygen
  • Hydrogen
  • Fermentation
  • Partial Oxygen Pressure
  • Medial Effect