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Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis

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Abstract

A specific recombinant antigen was evaluated for its immunodiagnostic potential in an enzyme-linked immunosorbent assay (ELISA). The antigen was used as a glutathione S-transferase (GST) fusion protein (DvGST3-14) or as a pure recombinant parasite protein (Dv3-14). A total of 55 sera from cattle experimentally infected withDictyocaulus viviparus, 24 sera from naturally infected cattle and 25 sera from helminth-free cattle were examined. ELISA results obtained for the sera from experimental infections showed a calculated specificity of >99% for both antigen preparations had a sensitivity of 93% (DvGST3-14) and 91% (Dv3-14). For field sera from natural infections, the specificity was calculated to be 90% (DvGST3-14) and >99% (Dv3-14) and the sensitivity, 85% (DvGST3-14) and 70% (Dv3-14).

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Dedicated to Prof. Dr. K.T. Friedhoff on the occasion of his 60th birthday

This work was supported by grant Az Schn 267/4-1 from the Deutsche Forschungsgemeinschaft

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Schnieder, T. Use of a recombinantDictyocaulus viviparus antigen in an enzyme-linked immunosorbent assay for immunodiagnosis of bovine dictyocaulosis. Parasitol Res 78, 298–302 (1992). https://doi.org/10.1007/BF00937087

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Keywords

  • Glutathione
  • Fusion Protein
  • Immunosorbent Assay
  • Experimental Infection
  • Natural Infection