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Phosphorylation ofToxoplasma gondii major surface antigens

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Abstract

The phosphorylation of the major surface proteins ofToxoplasma gondii tachyzoites was investigated. Metabolic labeling of intracellular tachyzoites with [32P]-orthophosphate followed by immunoprecipitation with specific monoclonal antibodies showed that all five major surface proteins were labeled as expected for GPI-anchored proteins. More detailed analysis of the phosphorylated sites revealed that surface proteins P30 and P22 contained phosphoserine residues in addition to the phosphorylated molecules that are presumably localized in the GPI-membrane anchors

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Abbreviations

BSA:

bovine serum albumin

GPI:

glycosyl-phosphatidylinositol

mAb:

monoclonal antibody

NP-40:

Nonidet P-40

PBS:

phosphate-buffered saline

PMSF:

phenylmethylsulfonylfluoride

TCA:

trichloroacetic acid

References

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Author information

Correspondence to Jean François Dubremetz.

Additional information

This work was supported by the Institut National de la Santé et de la Recherche Médicale (INSERM)

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Tomavo, S., Martinage, A. & Dubremetz, J.F. Phosphorylation ofToxoplasma gondii major surface antigens. Parasitol Res 78, 541–544 (1992). https://doi.org/10.1007/BF00936449

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Keywords

  • Orthophosphate
  • Monoclonal Antibody
  • Detailed Analysis
  • Surface Protein
  • Surface Antigen