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Effects of reactive oxygen intermediate scavengers on the antitoxoplasmic activity of activated macrophages

Abstract

Obioactin, Lonomycin A, muramyl dipeptide, and scavengers of hydrogen radicals and of singlet oxygen were used to study the participation of ·OH and1O2 in the killing ofToxoplasma in cultures of glycogen-induced peritoneal macrophages. Both the scavengers of OH (diazabicyclooctane and histidine) and those of1O2 (mannitol and sodium benzoate) failed to inhibit the multiplication ofToxoplasma in macrophages that were incubated with either Obioactin, Lonomycin A, or MDP. The results of these experiments demonstrate the apparent lack of an inhibitory effect of ·OH and1O2 on the multiplication ofToxoplasma, whereas the scavengers alone inhibited the growth of the parasites.

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Correspondence to A. Saito.

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Saito, A., Igarashi, I., Miyahara, K. et al. Effects of reactive oxygen intermediate scavengers on the antitoxoplasmic activity of activated macrophages. Parasitol Res 78, 28–31 (1992). https://doi.org/10.1007/BF00936177

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Keywords

  • Oxygen
  • Hydrogen
  • Sodium
  • Histidine
  • Mannitol