A non-axenic and an axenic isolate ofBlastocystis hominis have been induced to form cysts in vitro using an encystation medium. The morphology of the parasite at different time points was observed by scanning electron microscopy. In day-2 cultures the cysts were spherical and had a non-uniform, coarse outer surface around the body. A deep, pore-like opening was seen in some of the parasites. Most of the cysts from day-4 and day-6 cultures ruptured, revealing small, uniformly sized spherical bodies occurring in grape-like clusters. Acridine orange staining confirmed that these bodies were the progeny ofBlastocystis hominis. A multiple fission-like reproduction process giving rise to many daughterBlastocystis occurs within the cyst.
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Suresh, K., Howe, J., Ng, G.C. et al. A multiple fission-like mode of asexual reproduction inBlastocystis hominis . Parasitol Res 80, 523–527 (1994). https://doi.org/10.1007/BF00932701
- Electron Microscopy
- Scanning Electron Microscopy
- Outer Surface
- Acridine Orange