Hepatic, pulmonary, and intestinal granuloma formation was comparatively studied in mice implanted with freshly laidSchistosoma japonicum eggs. The liver and the lung showed similar kinetics of tissue reactivity, with the magnitude in the lung being of a significantly lower degree. When the footpad-swelling test was performed, the delayed-type hypersensitivity (DTH) response during hepatic granuloma formation was found to be significantly increased from the 28th day after egg implantation onward, whereas pulmonary granuloma formation showed a peak response at 14–21 days, suggesting differing kinetics of granulomatous reaction. In histologic analysis, the temporal infiltration of monocytes was revealed to correspond to the increase in the DTH response. During intestinal granuloma formation, cosinophil infiltration was the most marked feature. The present study demonstrates that throughout the course of reaction, cellular components participating in egg-granuloma formation differ greatly according to the tissues involved.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Amano T, Oshima T (1987) Granuloma formation around inoculatedSchistosoma japonicum eggs in the livers of ddY mouse. Jpn J Parasitol 36:128–134
Cheever AW, Deb S, Duvall RH (1989) Granuloma formation inSchistosoma japonicum infected nude mice: the effects of reconstitution with L3T4+ or Lyt2+ splenic cells. Am J Trop Med Hyg 40:66–71
Cornu JA, Louis D, Grimaud JA (1978) Polyvinylchloride (PVC) particle implantation in mouse liver. A technique for experimental study of schistosome egg induced liver pathology. Experientia 34:547–549
Edungbola LD, Schiller EL (1979) Histopathology of hepatic and pulmonary granulomata experimentally induced with eggs ofSchistosoma mansoni. J Parasitol 65:253–261
Edungbola LD, Cha YN, Bueding E, Schiller EL (1982) Granuloma formation around exogenous eggs ofSchistosoma mansoni andSchistosoma japonicum in mice. Afr J Med Sc 11:75–79
Garb KS, Stavitsky AB, Mahmoud AAF (1981) Dynamics of antigen-and mitogen-induced responses in murine schistosomiasis japonica: in vitro comparison between hepatic granulomas and splenic cells. J Immunol 127:115–120
Hirata M, Hieda T, Tsutsumi H, Shimada M, Sato K, Koech DV, Wambayi E (1986) Immunodiagnosis of schistosomiasis haematobia using eggs collected from human urine as antigen. Jpn J Parasitol 35:437–443
Hirata M, Takushima M, Kage M, Fukuma T (1991) Induction of experimental murine granuloma formation againstSchistosoma japonicum eggs produced by in vitro ova deposition, in vitro tissue extraction, or lyophilization. Parasitol Res 77:315–319
Hirata M, Kage M, Takushima M, Fukuma T (1993) Different courses of granulomatous reactions aroundSchistosoma japonicum eggs in 3 strains of mice. J Parasitol (in press)
Ishii A, Owhashi M, Hashiguchi J (1982)Schistosoma japonicum: immediate hypersensitivity reactions to egg antigen in humans and rodents. Exp Parasitol 54:104–112
Lichtenberg F von (1962) Host response to eggs ofSchistosoma mansoni: 1. Granuloma formation in the unsensitized laboratory mouse. Am J Pathol 41:711–731
Olds GR, Mahmoud AAF (1981) Kinetics and mechanisms of pulmonary granuloma formation aroundSchistosoma japonicum eggs injected into mice. Cell Immunol60:251–260
Stavitsky AB (1987) Immune regulation in schistosomiasis japonica. Immunol Today 8:228–233
Warren KS, Boros DL, Hang LM, Mahmoud AAF (1975) TheSchistosoma japonicum egg granuloma. Am J Pathol 80:279–293
Weinstock JV, Boros DL (1981) Heterogeneity of the granulomatous response in the liver, colon, ileum, and ileal Peyer's patches to schistosome eggs in murine schistosomiasis mansoni. J Immunol 127:1906–1909
Weinstock JV, Boros DL (1983) Organ-dependent differences in composition and function observed in hepatic and intestinal granulomas isolated from mice with schistosomiasis mansoni. J Immunol 130:418–422
About this article
Cite this article
Hirata, M., Takushima, M., Kage, M. et al. Comparative analysis of hepatic, pulmonary, and intestinal granuloma formation around freshly laidSchistosoma japonicum eggs in mice. Parasitol Res 79, 316–321 (1993). https://doi.org/10.1007/BF00932188
- Comparative Analysis
- Lower Degree
- Histologic Analysis
- Cellular Component
- Tissue Reactivity