Immunoscreening of an adultSchistosoma mansoni cDNA expression library, using antibodies raised against purified adult worm tegumental surface membranes, identified a recombinant clone containing a 141-bp insert. Antibodies raised against the recombinant antigen bound specifically to the tegument of adult worms and immunoprecipitated the major 25000-dalton surface membrane antigen as well as a 22000-dalton nascent polypeptide generated by cell-free translation of adultS. mansoni mRNA. The mature 25000-dalton antigen was found to be precipitated by antibodies from infected mice, rats and humans.
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sodium dodecyl sulphate
polyacrylamide gel electrophoresis
serum from mice vaccinated with highly irradiated cercariae
serum from chronically infected mice
rabbit anti-membrane serum
infected rat serum
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Knight, M., Kelly, C., Rodrigues, V. et al. A cDNA clone encoding part of the major 25000-dalton surface membrane antigen of adultSchistosoma mansoni . Parasitol Res 75, 280–286 (1989). https://doi.org/10.1007/BF00931812
- cDNA Clone
- Surface Membrane
- Infected Mouse
- Adult Worm