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A cDNA clone encoding part of the major 25000-dalton surface membrane antigen of adultSchistosoma mansoni

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Abstract

Immunoscreening of an adultSchistosoma mansoni cDNA expression library, using antibodies raised against purified adult worm tegumental surface membranes, identified a recombinant clone containing a 141-bp insert. Antibodies raised against the recombinant antigen bound specifically to the tegument of adult worms and immunoprecipitated the major 25000-dalton surface membrane antigen as well as a 22000-dalton nascent polypeptide generated by cell-free translation of adultS. mansoni mRNA. The mature 25000-dalton antigen was found to be precipitated by antibodies from infected mice, rats and humans.

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Abbreviations

IPTG:

isopropylthio-beta-galactoside

SDS:

sodium dodecyl sulphate

PAGE:

polyacrylamide gel electrophoresis

PMSF:

phenylmethylsulphonyl fluoride

FTTC:

fluorescein isothiocyanate

VMS:

serum from mice vaccinated with highly irradiated cercariae

CMS:

serum from chronically infected mice

Ram:

rabbit anti-membrane serum

IRS:

infected rat serum

References

  1. Ardeshir F, Flint JE, Reese RT (1985) Expression ofPlasmodium falciparum surface antigens inEscherichia coli. Proc Natl Acad Sci USA 82:2518–2522

  2. Bickle QD, Andrews BS, Taylor MG (1986)Schistosoma mansoni: characterization of two protective monoclonal antibodies. Parasitol Immunol 8:95–107

  3. Fraker PJ, Speck JC Jr (1978) Protein and cell membrane iodination with a sparingly soluble chloramide, 1,3,4,6-tetrachloro-3a,6a-diphenylglycoluril. Biophys Biochem Res Commun 80:849–857

  4. Kelly C (1987) Molecular studies of schistosome immunity. In: Rollinson D, Simpson AJG (eds) The biology of schistosomes from genes to latrines. Academic Press, London, pp 265–293

  5. Kelly C, Knight M, Simpson AJG, Hackett F, Geisow MJ, Smithers SR (1987) Purification and amino acid sequencing ofSchistosoma mansoni surface antigens. UCLA Symposia on Molecular and Cellular Biology, New Seres, vol 60, pp 257–266

  6. Knight M, Simpson AJG, Payares G, Chaudhri M, Smithers SR (1984) Cell-free synthesis ofSchistosoma mansoni surface antigens: stage-specificity of their expression. EMBO J 3:213–219

  7. Knight M, Simpson AJG, Bickle Q, Hagan P, Moloney A, Wilkins A, Smithers SR (1986) Adult schistosome cDNA libraries as a source of antigens for the study of experimental and human schistosomiasis. Mol Biochem Parasitol 18:235–253

  8. Knopf PM, Cioli D (1980)Schistosoma mansoni: resistance to an infection with cercariae induced by the transfer of live adult worms to the rat. Int J Parasitol 10:13–21

  9. Laemmli UK (1971) Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680–685

  10. O'Farrel PH (1975) High resolution two-dimensional gel electrophoresis of proteins. J Biol Chem 250:4007–4021

  11. Payares G, Evans WH (1987) Surface proteins and antigens of adultSchistosoma mansoni tegumental membranes detached onto poly-lysine coated beads. Mol Biochem Parasitol 23:129–137

  12. Payares G, McLaren DJ, Evans WH, Smithers SR (1985) Antigenicity and immunogenicity of the outer membrane of adultSchistosoma mansoni. Parasitol Immunol 7:45–61

  13. Sanger F, Nicklen S, Coulson AR (1977) Sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74:5436–5467

  14. Simpson AJG, Schryer MD, Cesari IM, Evans WH, Smithers SR (1981) Isolation and partial characterisation of the tegumental outer membrane of adultSchistosoma mansoni. Parasitology 83:163–177

  15. Smithers SR, Terry RJ (1965) The infection of laboratory hosts with cercariae ofSchistosoma mansoni and the recovery of the adult worms. Parasitology 55:695–700

  16. Smithers SR, Terry RJ (1969) Immunity in Schistosomiasis. Ann NY Acad Sci 160:826–840

  17. Tavares CAP, de Rossi R, Payares G, Simpson AJG, McLaren DJ, Smithers SR (1984) A monoclonal antibody raised against adultSchistosoma mansoni which recognizes a surface antigen on schistosomula. Z Parasitenkd 70:189–197

  18. Voller A, Bidwell DE, Bartlet A (1979) The enzyme linked immunosorbent assay (ELISA). Guensey GB (ed) Dynatech, Europe

  19. Webbe G, James C, Nelson GS, Smithers SR, Terry RJ (1976) Acquired resistance toSchistosoma haematobium in the baboon (Papio anubis) after cercarial exposure and adult worm transplantation. Ann Trop Med Parasitol 70:411–424

  20. Yi X, Omer Ali P, Kelly C, Simpson AJG, Smithers SR (1986) IgM antibodies recognising epitopes shared between schistosomula and miracidia ofSchistosoma mansoni which block in vitro killing. J Immunol 137:3946–3954

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Correspondence to A. J. G. Simpson.

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Knight, M., Kelly, C., Rodrigues, V. et al. A cDNA clone encoding part of the major 25000-dalton surface membrane antigen of adultSchistosoma mansoni . Parasitol Res 75, 280–286 (1989). https://doi.org/10.1007/BF00931812

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Keywords

  • Polypeptide
  • cDNA Clone
  • Surface Membrane
  • Infected Mouse
  • Adult Worm