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A cDNA clone encoding part of the major 25000-dalton surface membrane antigen of adultSchistosoma mansoni

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Immunoscreening of an adultSchistosoma mansoni cDNA expression library, using antibodies raised against purified adult worm tegumental surface membranes, identified a recombinant clone containing a 141-bp insert. Antibodies raised against the recombinant antigen bound specifically to the tegument of adult worms and immunoprecipitated the major 25000-dalton surface membrane antigen as well as a 22000-dalton nascent polypeptide generated by cell-free translation of adultS. mansoni mRNA. The mature 25000-dalton antigen was found to be precipitated by antibodies from infected mice, rats and humans.

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sodium dodecyl sulphate


polyacrylamide gel electrophoresis


phenylmethylsulphonyl fluoride


fluorescein isothiocyanate


serum from mice vaccinated with highly irradiated cercariae


serum from chronically infected mice


rabbit anti-membrane serum


infected rat serum


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Correspondence to A. J. G. Simpson.

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Knight, M., Kelly, C., Rodrigues, V. et al. A cDNA clone encoding part of the major 25000-dalton surface membrane antigen of adultSchistosoma mansoni . Parasitol Res 75, 280–286 (1989). https://doi.org/10.1007/BF00931812

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  • Polypeptide
  • cDNA Clone
  • Surface Membrane
  • Infected Mouse
  • Adult Worm