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High osmotic pressure forPneumocystic carinii London Resin White embedding enables fine immunocytochemistry studies: I. Golgi complex and cell-wall synthesis

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A method for embeddingPneumocystis carinii in hydrophilic resin (London Resin White) has been developed for immunocytochemistry studies. Using high osmotic pressure (about 850 mosmol) from fixation to embedding, this method improved the preservation of the fine structure as well as the antigenicity of rabbit-and SCID mouse-derivedP. carinii. Cytochemistry studies were performed using colloidal gold-conjugated lectins (concanavalin A, glycine max,Ulex europaeus) that reacted with the cytoplasmic components (endoplasmic reticulum, Golgi vesicles). Colloidal gold-conjugated streptavidin was also tested and was found to be reactive with the parasite cell wall and cytoplasmic components, which precludes its indiscriminate use inP. carinii immunocytochemistry studies.

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Correspondence to F. Palluault.

Additional information

This investigation was supported in part by the Agence Nationale de Recherche sur le SIDA (ANRS). The senior author (F.P.) was awarded a scholarship from the Conseil Régional Nord-Pas de Calais

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Palluault, F., Soulez, B., Slomianny, C. et al. High osmotic pressure forPneumocystic carinii London Resin White embedding enables fine immunocytochemistry studies: I. Golgi complex and cell-wall synthesis. Parasitol Res 78, 482–488 (1992).

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  • Cell Wall
  • Glycine
  • Endoplasmic Reticulum
  • Fine Structure
  • Osmotic Pressure