Drug-resistant cancer cells with the multidrug-resistance phenotype show overexpression ofP-glycoprotein, and we therefore tested carcinoma tissue from five patients with stage III or IV ovarian cancer forP-glycoprotein using 265/F4 and C 219 monoclonal antibodies, prepared against membrane glycoproteins in colchicine-resistant CHO cells. Using immunofluorescence and immunoblotting techniques, one of the tumors showed a positive reaction. Using the pcDR 1.5 clone we found that the same cancer tissue had elevated expression of the genes responsible for multidrug resistance. The demonstration of elevatedP-glycoprotein in ovarian carcinomas indicates thatP-glycoprotein overexpression is not limited to experimental tumor models.
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Volm, M., Efferth, T., Bak, M. et al. Detection of drug resistance in human ovarian carcinoma. Arch Gynecol Obstet 244, 123–128 (1989). https://doi.org/10.1007/BF00931383
- Human ovarian carcinoma
- Multidrug resistance
- Monoclonal antibodies