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A new method for isolation of the intraerythrocytic stages ofPlasmodium andBabesia from their host cells

Abstract

A new method for the isolation of intraerythrocytic stages ofPlasmodium berghei andBabesia divergens from red blood cells is described. The technique is based on hydrodynamic forces occurring in a flow channel containing a turbulent liquid current, which are capable of rupturing infected erythrocytes and removing their plasma membrane from the parasites' surface. The temperature and the concentration of cells were revealed as factors influencing the hydrodynamic forces. About 90% of the intact and apparently infectious parasites of both species were isolated from the lysed erythrocytes.

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Correspondence to Heinz Mehlhorn.

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Sobolewski, B., Mackenstedt, U. & Mehlhorn, H. A new method for isolation of the intraerythrocytic stages ofPlasmodium andBabesia from their host cells. Parasitol Res 79, 33–41 (1993). https://doi.org/10.1007/BF00931215

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Keywords

  • Plasma Membrane
  • Blood Cell
  • Host Cell
  • Flow Channel
  • Hydrodynamic Force