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Differentiation ofSarcocystis species in European sheep by isoelectric focusing

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Abstract

Isoelectric focusing was carried out on total proteins derived from cystozoites ofSarcocystis tenella, S. arieticanis andS. gigantea. The overall protein profile of each species was distinct and reproducible and enabled the unequivocal differentiation of the threeSarcocystis species. In total, approximately 60 bands could be counted forS. tenella andS. arieticanis and approximately 45 bands forS. gigantea cystozoites in the pH range of 3–9. Isoelectric points (pIs) were determined for dominant peaks of densitometric scans of gels in the pH range of 3–9 (cathodal and anodal sample application) and arbitrary identification numbers were assigned to 25 (5 and 20) peaks ofS. tenella, 25 (8 and 17) peaks ofS. arieticanis and 21 (7 and 14) peaks ofS. gigantea; similar assignments were also used for 11 peaks ofS. tenella, 18 peaks ofS. arieticanis and 12 peaks ofS. gigantea observed on densitometric scans of gels in the pH range of 4–6.5.

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Tenter, A.M., Johnson, M.R. & Zimmerman, G.L. Differentiation ofSarcocystis species in European sheep by isoelectric focusing. Parasitol Res 76, 107–114 (1989). https://doi.org/10.1007/BF00930830

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Keywords

  • Total Protein
  • Isoelectric Point
  • Protein Profile
  • Identification Number
  • Dominant Peak