Isoelectric focusing was carried out on total proteins derived from cystozoites ofSarcocystis tenella, S. arieticanis andS. gigantea. The overall protein profile of each species was distinct and reproducible and enabled the unequivocal differentiation of the threeSarcocystis species. In total, approximately 60 bands could be counted forS. tenella andS. arieticanis and approximately 45 bands forS. gigantea cystozoites in the pH range of 3–9. Isoelectric points (pIs) were determined for dominant peaks of densitometric scans of gels in the pH range of 3–9 (cathodal and anodal sample application) and arbitrary identification numbers were assigned to 25 (5 and 20) peaks ofS. tenella, 25 (8 and 17) peaks ofS. arieticanis and 21 (7 and 14) peaks ofS. gigantea; similar assignments were also used for 11 peaks ofS. tenella, 18 peaks ofS. arieticanis and 12 peaks ofS. gigantea observed on densitometric scans of gels in the pH range of 4–6.5.
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Baldock FC, Thompson RCA, Kumaratilake LM (1985) Strain identification ofEchinococcus granulosus in determining origin of infection in a case of human hydatid disease in Australia. Trans R Soc Trop Med Hyg 79:238–241
Bogucki MS, Onodera M, Rosen NL, Lifter J, Hotez PJ, Königsberg WH, Richards FF (1982)Trypanosoma congolense: surface glycoproteins of two early bloodstream variants: III. Immunochemical characterization. Exp Parasitol 53:1–10
Bylund G, Djupsund BM (1977) Protein profiles as an aid to taxonomy in the genusDiphyllobothrium. Z. Parasitenkd 51:241–247
Dixon BR, Arai HP (1987) An investigation of host influence of soluble protein banding profiles ofHymenolepis spp. (Cestoidea), using isoelectric focusing. Can J Zool 65:2471–2474
Dubey JP, Speer CA, Fayer R (1989) Sarcocystosis of animals and man. CRC, Boca Raton, Florida
Ebert F (1982) The use of isoelectric focusing in thin layer polyacrylamide and agarose gels as a method for the characterization of VenezuelanTrypanosoma cruzi stocks. Tropenmed Parasitol 33:63–67
Heydorn AO (1985) Zur Entwicklung vonSarcocystis arieticanis n. sp. Berl Münch Tierärztl Wochenschr 98:231–241
Johnson MR, Hoberg EP (1989) Differentiation ofMoniezia expansa andMoniezia benedeni (Eucestoda: Cyclophyllidae) by isoelectric focusing. Can J Zool (in press)
Kumaratilake LM, Thompson RCA (1984) Biochemical characterisation of Australian strains ofEchinococcus granulosus by isoelectric focusing of soluble proteins. Int J Parasitol 14:581–586
Olsson I, Axiö-Fredriksson UB, Degerman M, Olsson B (1988) Fast horizontal electrophoresis: I. Isoelectric focusing and polyacrylamide gel electrophoresis using PhastSystem. Electrophoresis 9(1):16–22
Peters T Jr, Westgard JO (1986) Evaluation of methods. In: Tietz NW (ed) Textbook of clinical chemistry. WB Saunders, Philadelphia, p 411
Reiter I, Mareis A (1986) Zur Differenzierung vonSarcocystis muris undS. dispersa-Infektionen der Maus mittels isoelektrischer Fokussierung und Immunoassays. Dtsch Tierärztl Wochenschr 93:433–437
Righetti PG, Drysdale JW (1979) Applications of IEF. In: Work TS, Work E (eds) Laboratory techniques in biochemistry and molecular biology. North-Holland, Amsterdam, pp 530–533, 558–560, 569
Tenter AM (1987) Comparison of enzyme-linked immunosorbent assay and indirect fluorescent antibody test for the detection of IgG antibodies toSarcocystis muris. Zentralbl Bakteriol Parasitenkd Infektionskr Hyg Abt Orig Reihe A 267:259–271
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Tenter, A.M., Johnson, M.R. & Zimmerman, G.L. Differentiation ofSarcocystis species in European sheep by isoelectric focusing. Parasitol Res 76, 107–114 (1989). https://doi.org/10.1007/BF00930830
- Total Protein
- Isoelectric Point
- Protein Profile
- Identification Number
- Dominant Peak