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Enzymology of ADP-ribose polymer synthesis


In this minireview, we summarize recent advances on the enzymology of ADP-ribose polymer synthesis. First, a short discussion of the primary structure and cloning of poly(ADP-ribose) polymerase (PARP) [EC], the enzyme that catalyzes, the synthesis of poly(ADP-ribose), is presented. A catalytic distinction between the multiple enzymatic activities of PARP is established. The direction of ADP-ribose chain growth as well as the molecular mechanism of the automodification reaction catalyzed by PARP are described. Current approaches to dissect ADP-ribose polymer synthesis into individual reactions of initiation, elongation and branching, as well as a partial mechanistic characterization of the ADP-ribose elongation reaction at he chemical level are also presented. Finally, recent developments in the catalytic characterization of PARP by site-directed mutagensis are also briefly summarized.

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Correspondence to Rafael Alvarez-Gonzalez.

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Alvarez-Gonzalez, R., Pacheco-Rodriguez, G. & Mendoza-Alvarez, H. Enzymology of ADP-ribose polymer synthesis. Mol Cell Biochem 138, 33–37 (1994).

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Key words

  • poly(ADP-ribose) polymerase
  • structure
  • chemistry
  • kinetics
  • automodification
  • mechanism(s)