Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Anion-exchange separation for neotropical trypanosomes: a preliminary trial and a description ofTrypanosoma devei from the tamarinSaguinus midas niger

  • 38 Accesses

  • 4 Citations

Abstract

Anion-exchange separation trials using DEAE-cellulose columns were performed with blood from two single species of marsupial and edentate, three species of rodent and single species of carnivore, primate, cayman and lizard.Trypanosoma cruzi was isolated fromDidelphis marsupialis, Dasypus novemcinctus andCoendou sp.T. (Megatrypanum) devei was isolated from the tamarinSaguinus midas niger and the mensural characters of the organism were redescribed. Anion-exchange separation was considered to be a valuable procedure for the taxonomist searching for new or little-known trypanosomes.

This is a preview of subscription content, log in to check access.

References

  1. Alvarenga NJ, Brener Z (1979) Isolation of pure metacyclic trypomastigotes ofTrypanosoma cruzi from triatomine bugs by use of a DEAE-cellulose column. J Parasitol 65:814–815

  2. De Souza W, Arguello C, Martinez-Palomo A, Trissl D, Gonzales-Robles A, Chiari E (1977) Surface charge ofTrypanosoma cruzi. Binding of cationized ferritin and measurement of cellular electrophoretic mobility. J Protozool 24:411–415

  3. Gibson WC, Marshall TF deC, Godfrey DG (1980) Numerical analysis of enzyme polymorphism: a new approach to the epidemiology and taxonomy of trypanosomes of the subgenusTrypanozoon. Adv Parasitol 18:175–246

  4. Godfrey DG, Lanham SM (1971) Diagnosis of Gambian trypanosomiasis in man by isolating trypanosomes from blood passed through DEAE-cellulose. Bull WHO 45:13–19

  5. Gutteridge WE, Cover B, Gaborak M (1978) Isolation of blood and intracellular forms ofTrypanosoma cruzi from rats and other rodents and preliminary studies of their metabolism. Parasitology 76:159–176

  6. Hoare CA (1972) The trypanosomes of mammals. Blackwell Scientific Publications, Oxford

  7. Hollingshead S, Pethica BA, Ryley JF (1963) The electrophoretic behaviour of some trypanosomes. Biochem J 89:123–127

  8. Kilgour V (1980)Trypanosoma: Intricacies of biochemistry, morphology and environment. Int J Biochem 12:325–332

  9. Lainson R (1977)Trypanosoma cecili n.sp., a parasite of the South American cayman,Caiman crocodilus crocodilus (Linnaeus 1758) (Crocodilia: Alligatoridae). In: Protozoology, vol. 3. Publ by the London School of Hygiene and Tropical Medicine, pp 87–93

  10. Lainson R, Shaw JJ, Fraiha H, Miles MA, Draper CC (1979) Chagas's disease in the Amazon basin: I.Trypanosoma cruzi infections in silvatic mammals, triatomine bugs and man in the State of Pará, north Brazil. Trans R Soc Trop Med Hyg 73:193–204

  11. Lanham SM (1968a) Separation of trypanosomes from the blood of infected rats and mice by anion-exchangers. Nature 218:1273–1274

  12. Lanham SM (1968b) The separation of trypanosomes from blood cells and their behaviour on TEAE- and DEAE-cellulose and on DEAE-Sephadex. Trans R Soc Trop Med Hyg 62:129–130

  13. Lanham SM (1971) The separation of stercorarian trypanosomes from infected blood using DEAE-cellulose. Trans R Soc Trop Med Hyg 65:248–249

  14. Lanham SM, Godfrey DG (1970) Isolation of salivarian trypanosomes from man and other mammals using DEAE-cellulose. Exp Parasitol 28:521–534

  15. Lanham SM, Williams JE, Godfrey DG (1972) Detection of low concentrations of trypanosomes in blood by column separation and membrane filtration. Trans R Soc Trop Med Hyg 66:624–627

  16. Lanham SM, Miles MA, Shaw JJ, Lainson R (1981)Trypanosoma vivax in water buffalo (Bubalus bubalis) of the Amazon basin and the diagnosis of subpatent infection by anion exchange separation. Trans R Soc Trop Med Hyg 75:471–473

  17. Lumsden WHR, Kimber CD, Evans DA, Doig SJ (1979)Trypanosoma brucei: miniature anion-exchange centrifugation technique for detection of low parasitaemias: adaptation for field use. Trans R Soc Trop Med Hyg 73:312–317

  18. Lumsden WHR, Kimber CD, Dukes P, Haller L, Stanghellini A, Duvallet G (1981) Field diagnosis of sleeping sickness in the Ivory Coast. I. Comparison of the miniature anionexchange/centrifugation technique with other protozoological methods. Trans R Soc Trop Med Hyg 75:242–250

  19. Marinkelle CJ (1976) The biology of the trypanosomes of non-human primates. In: Lumsden WHR and Evans DA (eds) Biology of the kinetoplastida, vol 1. Academic Press, London, pp 217–256

  20. Maxie MG, Tabel H, Losos GJ (1978) Determination of volumes ofTrypanosoma vivax andT. congolense separated from cattle blood. Z Tropenmed Parasitol 29:234–238

  21. Mercado TI, Katusha K (1979) Isolation ofTrypanosoma cruzi from the blood of infected mice by column chromatography. Prep Biochem 9:97–106

  22. Miles MA, Behbehani MK, Lumsden WHR (1970) Accuracy of infectivity of titrations of trypanosome suspensions enhanced by anion-exchange separation. Trans R Soc Trop Med Hyg 64:15

  23. Miles MA, Lanham SM, Souza AA de, Póvoa MM (1980) Further enzymic characters ofTrypanosoma cruzi and their evolution for strain characterization. Trans R Soc Trop Med Hyg 74:221–237

  24. Miles MA, Póvoa MM, Souza AA de, Lainson R, Shaw JJ, Ketteridge DS (1981) Chagas's disease in the Amazon basin: II. The distribution ofTrypanosoma cruzi zymodemes 1 and 3 in Pará State, north Brazil. Trans R Soc Trop Med Hyg 75:667–674

  25. Murray PK, Boltz RC, Schmatz DM (1982) Separation of individual stages ofTrypanosoma cruzi grown in cell culture by continuous free flow electrophoresis. J Protozool 29:109–113

  26. Schmatz DM, Murray PK (1981)Trypanosoma cruzi: Selective isolation of pure trypomastigotes from cultured muscle cells. J Parasitol 67:517–521

  27. Villalta FV, Leon W (1979) Effect of purification by DEAE-cellulose column on infectivity ofTrypanosoma cruzi blood forms. J Parasitol 65:188–189

  28. Walker EP (1975) Mammals of the world, 3rd edn. Johns Hopkins University Press, Baltimore London

Download references

Author information

Correspondence to Sheila M. Lanham.

Additional information

This work was carried out by the authors at the Instituto Evandro Chagas Fundação, SESP, Belém and was supported by the Ministry of Overseas Development of H.M. Government, the Wellcome Trust and Fundação SESP and UNDP/World Bank/WHO Special Programme for Research and Training in Tropical Diseases

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Lanham, S.M., Miles, M.A., de Souza, A.A.A. et al. Anion-exchange separation for neotropical trypanosomes: a preliminary trial and a description ofTrypanosoma devei from the tamarinSaguinus midas niger . Z. Parasitenkd. 70, 311–319 (1984). https://doi.org/10.1007/BF00927817

Download citation

Keywords

  • Single Species
  • Trypanosoma
  • Preliminary Trial
  • Trypanosoma Cruzi
  • Valuable Procedure