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Further studies on in vitro pairing ofEchinostoma revolutum (Trematoda) adults

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Various factors that influence in vitro pairing ofEchinostoma revolutum adults were studied. More worms paired in Locke's, Ringer's, and 0.85% NaCl solutions than in the defined medium NCTC 135. Also, more pairing occurred at 39 or 43°C than at 35°C. Echinostomes placed, 1, 3, or 5 cm apart in 5.5 cm diameter Petri dishes as well as those placed 2, 4, or 6 cm apart in 8.5 cm diameter Petri dishes paired; however, worms placed 8 cm apart in the latter dishes did not. Individuals maintained in Locke's solution at 20 or 39°C for 1 h after having been removed from the chicks paired less frequently than those used for experiments immediately after their removal from chicks. Worms showed more pairing in fresh Locke's solution than in this solution preconditioned by excretory-secretory products ofE. revolutum. Scanning electron microscopy revealed no differences between the tegument of fresh worms and of those maintained under experimental conditions for 2 h at 39°C.

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  1. Bone LW, Shorey HH (1977) Disruption of sex pheromone communication in a nematode. Science 197:694–695

  2. Eveland LK, Fried B, Cohen LM (1982)Schistosoma mansoni: Chemoattraction studies with adult worms in the presence and absence of barriers. Exper Parasitol 54:271–276

  3. Fried B, Leiby DA (1982) Intraspecific and interspecific pairing ofHaematoloechus medioplexus (Trematoda) andEchinostoma revolutum (Trematoda) adultsin vitro, and observations onH. medioplexus lipophilic excretory-secretory products. Parasitology 84:375–380

  4. Fried B, Roberts TM (1972) Pairing ofLeucochloridiomorpha constantiae (Mueller, 1935) (Trematoda)in vitro, in the chick and on the chorioallantois. J Parasitol 58:88–91

  5. Fried B, Robinson GA (1981) Pairing and aggregation ofAmblosoma suwaense (Trematoda: Brachylaimidae) metacercariaein vitro and partial characterization of lipids involved in chemo-attraction. Parasitology 82:225–229

  6. Fried B, Tancer RB, Fleming SJ (1980) In vitro pairing ofEchinostoma revolutum (Trematoda) metacercariae and adults, and characterization of worm products involved in chemoattraction. J Parasitol 66:1014–1018

  7. Fried B, Weaver LJ (1969) Exposure of chicks to the metacercaria ofEchinostoma revolutum (Trematoda). Proc Helminthol Soc Wash 36:153–156

  8. Fried B, Wilson DB (1981) Intraspecific and interspecific pairing ofEchinostoma revolutum (Trematoda) andZygocotyle lunata (Trematoda) adults in vitro. Proc Helminthol Soc Wash 48:243–245

  9. Paul J (1975) Cell and Tissue Culture. 5th edn. Churchill Livingstone, Edinburgh

  10. Sturkie P (1965) Avian Physiology. 2nd edn. Comstock Publishing Associates Ithaca

  11. Tate ME, Clelland RC (1957) Nonparametric and Shortcut Statistics. Interstate Printers and Publishers, Danville

  12. Wagner HM (1975) Principles of Operations Research. Prentice-Hall, Inc, Englewood Cliffs

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Correspondence to Bernard Fried.

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Fried, B., Pallone, A. Further studies on in vitro pairing ofEchinostoma revolutum (Trematoda) adults. Z. Parasitenkd. 70, 297–302 (1984).

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  • Electron Microscopy
  • Scan Electron Microscopy
  • Petri Dish
  • Diameter Petri Dish
  • Medium NCTC