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Electrophoretic variation of enzymes: A further marker for genetic studies of theEimeria

Summary

Embryo-adapted strains ofEimeria mivati andE. mivati var.diminuta, differing in their sensitivity to sulphaquinoxaline and electrophoretic mobilities of lactate dehydrogenase, were crossed.E. mivati was sulphaquinoxaline-resistant and characterised by an electrophoretic form of the enzyme denoted lactate dehydrogenase-1 whereasE. mivati var.diminuta was sulphaquinoxaline-sensitive and characterised by lactate dehydrogenase-6. Progeny recovered from the cross were passaged in embryonating eggs given sulphaquinoxaline and the (drug-resistant) parasites recovered were characterised by both lactate dehydrogenase-1 and lactate dehydrogenase-6. Controls showed that those parasites characterised by the recombinant phenotype of drug-resistance and lactate dehydrogenase-6 had been produced by the cross-fertilisation of gametes.

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References

  1. Jeffers, T.K.: Genetic transfer of anticoccidial drug resistance inEimeria tenella. J. Parasitol.60, 900–904 (1974)

  2. Jeffers, T.K.: Genetic recombination of precociousness and anticoccidial drug resistance inEimeria tenella. Z. Parasitenkd.50, 251–255 (1976)

  3. Joyner, L.P., Norton, C.C.: Transferred drug resistance inEimeria maxima. Parasitology71, 385–392 (1975)

  4. Joyner, L.P., Norton, C.C.: Further observations on the genetic transfer of drug resistance inEimeria maxima. Parasitology74, 205–213 (1977)

  5. Long, P.L.:Eimeria mivati: reproduction, pathogenicity and immunogenicity of a strain maintained in chick embryos by serial passage. J. Comp. Pathol.82, 439–445 (1972)

  6. Long, P.L., Millard, B.J.:Eimeria infection of chicken embryos: the effect of known anticoccidial drugs againstE. tenella andE. mivati. Avian Pathol.2, 111–125 (1973)

  7. Rollinson, D.: Further electrophoretic studies on species ofEimeria. Parasitology73, (Abstract), IV (1976)

  8. Shirley, M.W.: Enzyme variation inEimeria species of the chicken. Parasitology71, 369–376 (1975)

  9. Shirley, M.W., Millard, B.J., Long, P.L.: Studies on the growth, chemotherapy and enzyme variation ofEimeria acervulina var.diminuta andE. acervulina var.mivati. Parasitology75, 165–176 (1977)

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Shirley, M.W. Electrophoretic variation of enzymes: A further marker for genetic studies of theEimeria . Z. Parasitenkd. 57, 83–87 (1978). https://doi.org/10.1007/BF00927630

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Keywords

  • Lactate
  • Genetic Study
  • Electrophoretic Mobility
  • Electrophoretic Variation
  • Recombinant Phenotype