Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Neoplastic association of enhanced type V collagen production in rat fibrosarcoma

  • 24 Accesses

  • 6 Citations

Abstract

Collagens present in the connective tissues of the extracellular matrix of fibrosarcoma were isolated and characterized. The fibrosarcoma was induced in rats by the administration of 3-methylcholanthrene. The results obtained were compared with normal muscle. An excess amount of type V collagen was found to be produced by the fibrosarcoma tissue compared to the normal muscle. Type V collagen from fibrosarcoma was characterized on the basis of solubility behavior in sodium chloride solutions, electrophoretic mobility on SDS-polyacrylamide gels, elution pattern of phosphocellulose chromatography and amino acid composition.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Krieg T, Hein A, Hatamochi A, Aumailley M: Molecular and clinical aspects of connective tissue. 18: 105–123, 1988

  2. 2.

    Liotta LA: Tumor Extracellular matrix. Lab Invest 47: 112–113, 1982

  3. 3.

    Miller EJ: (1984) Chemistry of the collagens and their distribution. In: KA Piez, AH Reddi (eds) Extracellular Matrix Biochemistry. Elsevier Science Publishing Co., New York

  4. 4.

    Prockop DJ: Mutations in collagen genes as a cause of connective tissue diseases. New Engl J Med 326: 540–546, 1992

  5. 5.

    Stenn KS, Madri JA, Roll FJ: Migrating epidermis produces AB2 collagen and requires continuous collagen synthesis for moment. Nature 277: 229–232, 1979

  6. 6.

    Squir CA, Kammayr GA: The role of connective tissue in the maintenance of epithelial differentiation in the adult. Cell Tissue Res 230: 615–630, 1983

  7. 7.

    Hayakawa T, Hashimoto Y, Myokei T, Aoyama H, Izawa Y: Changes in type of collagen during the development of human post-burn hypertrophic scars. Clin Chim Acta 93: 119–125, 1979

  8. 8.

    Murata K, Motayama T, Kotak C: Collagen types in various layers of the human aorta and their changes with the atherosclerotic process. Atherosclerosis 60: 251–262, 1986

  9. 9.

    Ehrlich HP, White BS: The identification of αA and αB collagen chains in hypertrophic scar. Exp Mol Pathol 34: 1–8, 1981

  10. 10.

    Miller EJ, Rhodes RK: Preparation and characterization of different types of collagens. Methods in Enzymology 82: 33–45, 1982

  11. 11.

    Sage H, Bornstein P: Characterization of a normal collagen chain in human placenta and its relation to AB collagen. Biochemistry 18: 3815–3822, 1979

  12. 12.

    Hay ED: Extracellular matrix. J Cell Biol 91: 205s-223s, 1981

  13. 13.

    Liotta LA, Rao CN, Barsky SH: Tumor invasion and the extracellular matrix. Lab Invest 49: 636–649, 1982

  14. 14.

    Liotta LA: Tumor invasion and metastases. Role of the extracellular matrix. Rhodes memorial award lecture. Cancer Res 46: 1–7, 1986

  15. 15.

    Jones PA, DeClerk YA: Extracellular matrix destruction by invasive tumor cells. Cancer Metastasis Rev 1: 289–317, 1982

  16. 16.

    Liotta LA, Thorgeirsson UP, Garbasia SH: Role of collagenases in tumor cell invasion. Cancer Metastasis Rev 1: 277–288, 1982

  17. 17.

    Krieg T, Timpl R, Kurkinen M, Veheri A: Type III procollagen is the major collagenous component produced by a continuous rhabdomyosarcoma cell line. FEBS Lett 104: 405–409, 1979

  18. 18.

    Stern R, Wilczek J, Thrope WP, Rosenberg SA, Cannon G: Procollagen as markers for the cell of origin of human bone tumors. Cancer Res 40: 325–328, 1980

  19. 19.

    Alitalo K, Keski-Oja J, Veheri A: Extracellular Matrix proteins characterize human tumor cell lines. Int J Cancer 27: 755–761, 1981

  20. 20.

    Barksy SH, Rao CN, Groteindorst GR, Liotta LA: Increased content of type V collagen in desmoplsia of human beast carcinoma. Am J Pathol 108: 276–283, 1982

  21. 21.

    Iozzo RV, Bolender RP, Wight TN: Proteoglycan changes in the intercellular matrix of human colon carcinoma. Lab Invest 47: 124–138, 1982

  22. 22.

    Bano M, Zwiebel JA, Salomon DS, Kidwell WR: Detection and partial characterization of collagen synthesis stimulating activities in rat mammary adenosarcomas. J Biol Chem 258: 2729–2735, 1983

  23. 23.

    Ooshima A: Collagen aB chains: Increased proportion in human athresclerosis. Science 213: 666–668, 1981

  24. 24.

    Narayanan AS, Clagott LA, Page RC: Effect of inflammation on the distribution of collagen types I, III, IV and V and type I trimer and fibronectin in human gingivae. J Dent Res 64: 1111–1116, 1985

  25. 25.

    Peltonen J, Kahari L, Uitto J, Jimenez SA: Increased expression of type VI collagen genes in systemic sclerosis. Arthritis Rheum 33: 1829–1835, 1990

  26. 26.

    Paulus W, Sage EH, Liszka U, Iruela-Arispe, Jellinger K: Increased levels of type VIII collagen in human brain tumours compared to normal brain tissue and non-neoplastic ceribral disorders. Br J Cancer 63: 367–371, 1991

  27. 27.

    Asokan R, Chandrakasan G, Puvanakrishnan R, Dhar SC: Separation and evaluation of changing pattern of glycosaminoglycans in 3-methylcholanthrene induced fibrosarcoma. Neoplasma 36: 273–279, 1989

  28. 28.

    Neuman RE, Logan MA: The determination of collagen and elastin in tissues. J Biol Chem 186: 549–556, 1950

  29. 29.

    Laemmli UK: Cleavage and structural proteins during assembly of the head of bacteriophage T4 Nature 277: 680–685, 1970

  30. 30.

    Black C, Douglas DM, Tanzer ML: Separation of cynogen bromide peptides of collagen by means of high performance liquid chromatography. J Chromatography 190: 393–400, 1980

  31. 31.

    Bornstein P, Sage H: Structurally distinct collagen types. Ann Rev Biochem 49: 957–1003, 1980

  32. 32.

    Heinrikson RL, Meredith SC: Amino acid analysis by reverse phase high performance liquid chromatography: Precolunn derivitization with phenylisothiocyanate. Anal Biochem 136: 65–74, 1984

  33. 33.

    Narayanan AS, Page RC, Meyers DF: Characterization of collagen of diseased human gingiva. Biochemistry 19: 5037–5042, 1980

  34. 34.

    Rhodes RK, Miller EJ: Physicochemical characterization and molecular organization of the collagen A and B chains. Biochemistry 17: 3442–3448, 1978

  35. 35.

    Uitto J, Perejda J. Connective tissue disease. Molecular pathology of the extracellular matrix. Part III: 249–542, 1987

  36. 36.

    Narayanan AS, Page RC: Synthesis of type V collagen by fibroblasts derived from normal, inflamed and hyperplastic human connective tissues. Collagen Rel Res 5: 297–304, 1985

  37. 37.

    Yamamoto M, Sumiyoshi H, Nakagami K, Tahara E: Distribution of collagen types I, III and V in fibrotic and neoplastic human liver. Acta Pathol (Jap) 34: 77–86, 1984

  38. 38.

    Marian B, Danner MW: Skin tumor promotion is associated with increased type V collagen content in dermis. Carcinogenesis 8: 151–154, 1987

  39. 39.

    Leushner JR, Most MD: Glycoproteins on the surface of smooth muscle cells involved in their interaction with type V collagen. Can J Biochem Cell Biol 63: 1176–1182, 1985

  40. 40.

    Mumby SM, Raugi GJ, Bornstein P: Light microscopic immunolocalization of thrombospondin in human tissues. J Cell Biol 92: 343–349, 1982

  41. 41.

    Kerenyi T, Voss B, Rauterberg J, Fromme HG, Jellinek H, Hauss WH: Connective tissue proteins on the injured endothelium of the rat aorta. Exp Mol Pathol 43: 151–161, 1985

  42. 42.

    Mayne R, Vail MS, Miller EJ: Characterization of the collagen chains synthesized by cultured smooth muscle cells derived from rhesus monkey thoracic aorta. Biochemistry 17: 446–452, 1978

  43. 43.

    Madri JA, Furthmayr H: Isolation and tissue localization of type AB2 collagen from lung parenchyma. Am J Pathol 94: 323–330, 1979

  44. 44.

    Martinez-Hernanez A, Gay S, Miller EJ: Ultrastructural localization of type V collagen in rat kidney. J Cell Biol 92: 343–349, 1982

  45. 45.

    Grotendorst GR, Seppa HE, Kleinman HK, Martin GR: Attachment of smooth muscle cells to collagen and their migration toward platelet derived growth factor. Proc Natl Acad Sci USA 78: 3669–3672, 1981

Download references

Author information

Correspondence to G. Kesava Reddy.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Asokan, R., Reddy, G.K. & Dhar, S.C. Neoplastic association of enhanced type V collagen production in rat fibrosarcoma. Mol Cell Biochem 120, 25–32 (1993). https://doi.org/10.1007/BF00925981

Download citation

Key words

  • extracellular matrix
  • collagen
  • fibrosarcoma