Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Human T-lymphocyte subset production of immune (γ) interferon

  • 13 Accesses

  • 31 Citations


Human Tμ, Tγ, and Tφ lymphocyte subpopulations have the capacity to respond to phytohemagglutinin (PHA)in vitro with proliferation and the production of a pH 2 and heat-labile γ interferon. This occurs both when the subsets are isolated by direct rosetting techniques or by negative selection. Macrophages enhance the production of the γ interferon by each lymphocyte subset and do not themselves produce γ interferon in response to products of PHA-activated lymphocyte subsets. Thus our studies indicate that subpopulations of T lymphocytes known to differ with regard to morphology, surface receptors, RNA content, response to corticosteroids and X-irradiation, and other functional capabilities do not differ with regard to their capacity to produce γ interferon.

This is a preview of subscription content, log in to check access.


  1. 1.

    Epstein LB, Cline MJ, Merigan TC: The interaction of human macrophages and lymphocytes in the PHA stimulated production of interferon. J Clin Invest 50:744–753, 1971

  2. 2.

    Epstein LB, Cline MJ, Merigan TC: PPD stimulated interferon:In vitro macrophage lymphocyte interaction in the production of a mediator of cellular immunity. Cell Immunol 2:602–613, 1971

  3. 3.

    Epstein LB, Stevens DA, Merigan TC: Selective increase in lymphocyte interferon response to vaccinia antigen following revaccination. Proc Nat Acad Sci USA 69:2632–2636, 1972

  4. 4.

    Epstein LB, Kreth HW, Herzenberg LA: Fluorescence activated cell sorting of human T and B lymphocytes. II. Identification of the cell type responsible for interferon production and cell proliferation in response to mitogens. Cell Immunol 12:407–421, 1974

  5. 5.

    Gupta S: Functionally distinct subpopulations of human T lymphocytes. A review. Clin Bull 8:100–107, 1978

  6. 6.

    Gupta S, Fernandes G, Nair M, Good RA: Spontaneous and antibody dependent cell-mediated cytotoxicity by human T cell subpopulations. Proc Nat Acad Sci USA 75:5137–5141, 1978

  7. 7.

    Goldblatt D, McManus NH, Epstein LB: A micromethod for preparation of human macrophage cultures for the study of lymphocyte-macrophage interaction in immune interferon production and blastogenesis. Immunopharm 1:13–20, 1978

  8. 8.

    Boyum A: Isolation of mononuclear cells and granulocytes from human blood. Scand J Clin Lab Invest 21 (Suppl 97):77–89, 1968

  9. 9.

    Gupta S, Good RA: Subpopulations of human T lymphocytes II. Effect of thymopoietin, corticosteroids, and irradiation. Cell Immunol 34:10–18, 1977

  10. 10.

    Gupta S, Good RA: Subpopulations of human T lymphocytes I. Studies in immunodeficient patients. Clin Exp Immunol 30:222–228, 1977

  11. 11.

    Frei J, Borel C, Horvath G, Cullity B., Vannotti A: Enzymatic studies in the different types of normal and leukemic human white cells. Blood 18:317–327, 1961

  12. 12.

    Epstein LB, McManus NH: Macro- and microassays for the antiviral effects of human and mouse interferons.In Manual of Clinical Immunology, 2nd ed, N Rose, H Friedman (eds). Washington, DC, Amer Soc Microbiol, 1980, pp 275–283

  13. 13.

    Epstein, LB: The comparative biology of immune and classical interferons.In Biology of the Lymphokines, S Cohen, E Pick, JJ Oppenheim (eds). New York, Academic Press, 1979, pp 443–514

  14. 14.

    Moretta L, Ferrarini M, Mingari MC, Moretta A, Webb SR: Subpopulations of human T cells identified by receptors for immunoglobulins and mitogen responsiveness. J Immunol 117:2171–2174, 1976

  15. 15.

    Grossi CE, Webb SR, Zicca A, Lydyard PM, Moretta L, Mingari MC, Cooper MD: Morphological and histochemical analysis of two human T cell subpopulations bearing receptors for IgM and IgG. J Exp Med 147:1405–1417, 1978

  16. 16.

    Andreeff M, Beck, JD, Darzynkiewicz Z, Traganos F, Gupta S, Melamed MR, Good RA: RNA content in human lymphocyte subpopulations. Proc Nat Acad Sci USA 75:1938–1942, 1978

  17. 17.

    Perussia B, Mangoni L, Engers HD, Trinchieri G: Interferon production by human and murine lymphocytes in response to alloantigens. J Immunol 125:1589–1595, 1980

  18. 18.

    Kapadia A, O'Reilly RJ, Good RA, Gupta S: Leukocyte migration inhibition factor (LMIF) production by human T cell subpopulation. Fed Proc 37:1365, 1978

  19. 19.

    Eardley DD, Shen FW, Gershon RK, Ruddle NH: Lymphotoxin production by subsets of T cells. J Immunol 124:1199–1202, 1980

  20. 20.

    Gupta S, Fernandes G, Rocklin R, Good RA: Histamine receptors on human T cell subsets.In New Trends in Human Immunology and Cancer Immunotherapy, B Serrou, C Rosenfeld (eds). Paris, Dion Editeur, 1980, pp 434–438

  21. 21.

    Hayward AR, Layward L, Lydyard PM, Moretta L, Dagg M, Lawton AR: Fc receptor heterogeneity of human suppressor T cells. J Immunol 121:1–5, 1978

  22. 22.

    Pichler WJ, Lum L, Broder S: Fc receptors on human T lymphocytes. I. Transition of Tγ to Tε cells. J Immunol 121:1540–1548, 1978

  23. 23.

    Schulof RS, Fernandes G, Good RA, Gupta S: Neuraminidase treatment of T lymphocytes. Effect on Fc Receptor phenotype and function. Clin Exp Immunol 40:611–619, 1980

  24. 24.

    Reinherz, EL, Moretta L, Roper M, Breard JM, Mingari MC, Cooper MD, Schlossman SF: Human T lymphocyte subpopulations defined by Fc receptors and monoclonal antibodies. A comparison. J Exp Med 151:969–974, 1980

  25. 25.

    Gupta S, Good RA: Subpopulations of human T lymphocytes: Laboratory and clinical studies. Immunol Rev 56:89–113, 1981

  26. 26.

    Kaszubowski PA, Husby G, Tung KSK, Williams RC Jr: T lymphocyte subpopulations in peripheral blood and tissues of cancer patients. Cancer Res 40:4648–4657, 1980

  27. 27.

    Epstein LB: The effects of interferons on the immune response in vitro and in vivo.In Interferons and Their Actions, WE Stewart II (ed). Cleveland, CRC Press, 1977, pp 92–132

  28. 28.

    Epstein LB: Mitogen and antigen induction of interferon in vitro and in vivo. Tex Rep Biol Med 35:42–56, 1977

  29. 29.

    Archer DL, Smith BG, Ulrich JT, Johnson HM: Immune interferon induction by T cell mitogens involves different T cell subpopulations. Cell Immunol 48:420–426, 1979

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Epstein, L.B., Gupta, S. Human T-lymphocyte subset production of immune (γ) interferon. J Clin Immunol 1, 186–194 (1981). https://doi.org/10.1007/BF00922762

Download citation

Key words

  • Human Tμ, Tγ, Tφ lymphocytes
  • γ interferon
  • macrophage-lymphocyte interaction