Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Variable effect of toxic shock toxins from different sources on neutrophil function in vitro

  • 20 Accesses

  • 3 Citations

Abstract

Toxic shock syndrome toxins (TSST) are 23–30 kD proteins that have been isolated from incubation media of strains ofStaphylococcus aureus cultured from patients with toxic shock syndrome (TSS). Injection of TSST into animals produces many of the symptoms that characterize TSS including shock, fever, and multiple organ failure. We found that addition of increasing concentrations of TSST-1-VP1035-16A, but not TSST-PEC, TSST-SEC, staphylococcal enterotoxin A or B, progressively decreased human neutrophil bactericidal activity againstS. aureus, 502A in vitro. TSST-1-VP1035-16A, but not the other toxins, also decreased superoxide anion and hydrogen peroxide concentrations in mixtures containing neutrophils and phorbol myristate acetate (PMA) in vitro. The results indicate that various preparations of TSST have different effects on neutrophil function in vitro and, accordingly, may have different effects in other in vitro and in vivo models of TSS.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Davis, J. P., P. J. Chesney, P. J. Wand, M. Laventure, and the Investigation and Laboratory Team. 1980. Toxic-shock syndrome: Epidemiologic features, recurrence, risk factors, and prevention.N. Engl. J. Med. 303:1429–1435.

  2. 2.

    Shands, K. N., G. P. Schmid, B. B. Dan, D. Blum, R. J. Guidotti, N. T. Hargrett, R. L. Anderson, D. L. Hill, C. F. Broome, J. D. Band, andD. W. Fraser. 1980. Toxic shock syndrome in menstruating women: Association with tampon use andStaphylococcus aureus and clinical features in 52 cases.N. Engl. J. Med. 303:1436–1442.

  3. 3.

    Osterholm, M. T., J. P. Davis, R. W. Gibson, J. S. Mandell, L. A. Wintermeyer, C. M. Helms, J. C. Forfang, J. Rondeau, J. M. Vergeront, and the Investigation Team. 1982. Tri-state toxic shock syndrome. I. Epidemiologie findings.J. Infect. Dis. 145:431–440.

  4. 4.

    Follow-up on toxic shock syndrome. 1980.MMWR 29(37):441–445.

  5. 5.

    Reingold, A. L., B. B. Dan, K. N. Shands, andC. W. Broome. 1982. Toxic shock syndrome not associated with menstruation: A review of 54 cases.Lancet 1:1–4.

  6. 6.

    Bergdoll, M. S., B. A. Crass, R. F. Reiser, R. N. Robbins, andJ. P. Davis. 1981. A new staphylococcal enterotoxin, enterotoxin F, associated with toxic shock syndromeStaphylococcus aureus isolates.Lancet 1:1017–1021.

  7. 7.

    Todd, J., M. Fishaut, F. Kapral, andT. Welch. 1978. Toxic shock syndrome associated with phage-group-1 staphylococci.Lancet 2:1116–1118.

  8. 8.

    Schlievert, P. M., K. N. Shands, B. B. Dan, G. P. Schmid, andR. D. Nishimura. 1981. Identification and characterization of an exotoxin fromStaphylococcus aureus associated with toxic shock syndrome.J. Infect. Dis. 143:509–516.

  9. 9.

    Cohen, M. L., andS. Falkow. 1981. Protein antigens fromStaphylococcus aureus strains associated with toxic shock syndrome.Science 211:842–844.

  10. 10.

    Bonventre, P. F., L. Weckbach, J. Staneck, P. M. Schlievert andM. Thompson. 1983. Production of Staphylococcal enterotoxin F and pyrogenic exotoxin C byStaphylococcal aureus isolates from toxic shock syndrome-associated sources.Infect. Immun. 40(3):1023–1029.

  11. 11.

    Bergdoll, M. S., andP. M. Schlievert. 1984. Toxic shock syndrome toxin.Lancer 2:691 (letter).

  12. 12.

    Chesney, P. J., M. S. Bergdoll, J. P. Davis, andJ. M. Vergeront. 1984. The disease spectrum, epidemiology and etiology of toxic-shock syndrome,Annu. Rev. Microbiol. 38:315–338.

  13. 13.

    Goetz, M. B., R. A. Proctor, R. F. Reiser, andM. S. Bergdoll. 1983. Effect ofStaphylococcus aureus enterotoxin F on human neutrophil oxidative metabolism.J. Infect. Dis. 148(4):764.

  14. 14.

    Hjorth, R., A. K. Jonsson, andP. Vretblad. 1981. A rapid method for purification of human granulocytes using Percoll. A comparison with dextran sedimentation,J. Immunol. Methods 43:95–101.

  15. 15.

    Repine, J. E., andC. C. Clawson. 1977. Quantitative measurement of the bactericidal capability of neutrophils from patients and carriers of chronic granulomatous diseases.J. Lab. Clin. Med. 90:522–528.

  16. 16.

    Berger, E. M., R. N. Harada, A. E. Vatter, C. M. Bowman, andJ. E. Repine. 1984. Cyclical abnormalities in the bactericidal function, Superoxide production and lysozome activity of neutrophils obtained from a healthy woman during menstruation: Reversal by pretreatment with aspirin.J. Infect. Dis. 149:413–410.

  17. 17.

    Cassell, R. H., andI. Fridovich. 1975. The role of Superoxide radical in the autooxidation of cytochromec.Biochemistry 14(9):1866–1868.

  18. 18.

    Thurman, R. G., H. G. Ley, andR. Scholz. 1972. Hepatic microsomal ethanol oxidation: Hydrogen peroxide formation and the role of catalase.Eur. J. Biochem. 25:420–430.

  19. 19.

    Weiss, S. J., J. Young, A. F. Lobuglio, A. F. Slivka, andA. Slivka. 1981. Role of hydrogen peroxide in neutrophil-mediated destruction of cultured endothelial cells.J. Clin. Invest. 68:714–721.

  20. 20.

    Decker, L. A., editor. 1977. Worthington Enzyme Manual. Worthington Biochemical, Freehold, New Jersey. 185–188.

  21. 21.

    Quimby. F., andH. T. Nguyen. 1985. Animal studies of toxic shock syndrome.CRC Crit. Rev. Microbiol 12:1–44.

  22. 22.

    Kushnaryov, V. M., M. S. Bergdoll, H. S. MacDonald, J. Vellinga, andR. Reiser. 1984. Study of staphylococcal toxic shock syndrome toxin in human epithelial cell culture.J. Infect. Dis. 150(4):535–545.

  23. 23.

    Schlievert, P. M. 1983. Alteration of immune function by staphylococcal pyrogenic exotoxin type C: Possible role in toxic-shock syndrome.J. Infect. Dis. 147:391–398.

  24. 24.

    Schlievert, P. M. 1984. Biological properties of toxic shock syndrome exotoxin.Surv. Synth. Pathol. Res. 3:54–62.

  25. 25.

    Klebanoff, S. J., andR. A. Clark. 1978. Anti-microbial systems.In The Neutrophil: Function and Clinical Disorders. North Holland Publishing, New York. 409–488.

  26. 26.

    Repine, J. E. 1985. Neutrophils, oxygen radicals and the adult respiratory distress syndrome.In The Pulmonary Circulation and Acute Lung Injury. Futura Publishing, Mount Kisco, New York. 249–280.

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Berger, E.M., Shibao, G.A., Brown, S.N. et al. Variable effect of toxic shock toxins from different sources on neutrophil function in vitro. Inflammation 12, 447–453 (1988). https://doi.org/10.1007/BF00919438

Download citation

Keywords

  • Incubation Medium
  • Phorbol
  • Variable Effect
  • Multiple Organ Failure
  • Human Neutrophil