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Effect of alpha-2-macroglobulin on cytokine-mediated human C-reactive protein production


Alpha-2-macroglobulin (α2-M), a serum protease inhibitor that also binds cytokines, neutralized the inhibitory effect exerted by transforming growth factor-Β (TGF-Β) on IL-6-induced C-reactive protein (CRP) production by the human hepatoma cell line PLC/PRF/5. α2-M was found to bind noncovalently with TGF-Β to form a complex that, upon acidification, released TGF-Β inhibitory activity as detected by IL-6-induced CRP production. Although α2-M also binds IL-6, it did not alter IL-6-induced CRP production by the hepatoma cells. The interaction between α2-M and TGF-Β may influence the production of acute-phase proteins by liver hepatocytes.

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Taylor, A.W., Mortensen, R.F. Effect of alpha-2-macroglobulin on cytokine-mediated human C-reactive protein production. Inflammation 15, 61–70 (1991).

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  • Public Health
  • Internal Medicine
  • Protease Inhibitor
  • Inhibitory Activity
  • Protein Production