Local and systemic chemiluminescent (CL) response was measured in 32 patients with wound infection and in 27 patients with crush syndrome complicated by severe wound infection. The level of wound CL depends upon the local microbial concentration and the dynamics of wound healing. CL response of neutrophils in circulation was higher in higher local CL response, but extremely high wound microbial concentration (> 108 CFU/g) suppressed both local and systemic responses. No metabolic reserve was observed in wound tissue. Wound healing was characterized by changes in the mode of local CL response: from a prevalence of luminol-dependent (neutrophilic) CL to a prevalence of lucigenin-independent (macrophagal) CL.
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Bordet, J. 1897. Infection streptococcique.Ann. Inst. Pasteur 11:177–213.
Krizek, T. J., andM. C. Robson. 1975. Evolution of quantitative bacteriology in wound management.Am. J. Surg. 130:579–584.
Agarwal, D. S. 1967. Subcutaneous staphylococcal infection in mice. II. The inflammatory response to different strains of staphylococci and micrococci.Br. J. Exp. Pathol. 48:469–482.
Burke, J. F., andA. A. Miles. 1958. The sequence of vascular events in early infective inflammation.J. Pathol. Bacteriol. 76:1–19.
Hart, P. H., L. K. Spencer, M. F. Nulsen, P. J. McDonald, andJ. J. Finlay-Jones. 1986. Neutrophil activity in abscess-bearing mice: comparative studies with neutrophils isolated from peripheral blood, elicited peritoneal exudates, and abscesses.Infect. Immun. 51:936–941.
Zimmerli, W., P. D. Lew, andF. A. Waldvogel. 1984. Pathogenesis of foreign body infection. Evidence for a local granulocyte defect.J. Clin. Invest. 73:1191–1200.
Belotsky, S. M., E. S. Dikovskaya, andV. G. Petukhov. 1987. Luminoldependent chemiluminescence of primary focus against different infective doses ofStaphylococcus aureus.Zurn. Microbiol. Epidemiol. Immunobiol. (Moscow),3:53–55.
Ryan, U. S. 1987. Endothelial cell activation responses.In Pulmonary Endothelium in Health and Disease. U. S. Ryan, editor. Dekker, New York. 3–33.
Shah, S. V., W. H. Baricos, andA. Basci. 1987. Degradation of human glomerular basement membrane by stimulated neutrophils. Activation of a metalloproteinase(s) by reactive oxygen metabolites.J. Clin. Invest. 79:25–31.
Vandenbroucke-Grauls, C. M. J. E., H. M. W. M. Thussen, K. P. M. van Kessel, B. S. van Asbeck, andJ. Verhoef. 1987. Injury to endothelial cells by phagocytosing polymorphonuclear leukocytes and modulatory role of lipoxygenase products.Infect. Immun. 55:1447–1454.
Vissers, M. C. M., W. A. Day, andC. C. Winterburn. 1985. Neutrophils adherent to a nonphagocytosable surface (glomerular basement membrane) produce oxidants only at the site of attachment.Blood 66:161–166.
Fantone, J. C., andP. A. Ward. 1985. Polymorphonuclear leukocyte-mediated cell and tissue injury. Oxygen metabolites and their relations to human disease.Hum. Pathol. 16:973–978.
Briheim, G., P. Follin, S. Sandstedt, andC. Dahlgren. 1989. Relationship between intracellularly and extracellularly generated oxygen metabolites from primed polymorphonuclear leukocytes differs from that obtained from nonprimed cells.Inflammation 13:455–464.
Belotsky, S. M., T. I. Snastina, O. B. Filjukova, andA. A. Zvyagin. 1988. Microbial factor in the chemiluminescence of peripheral blood neutrophils of patients with purulent surgical infection.Zurn. Microbiol. Epidemiol. Immunobiol. (Moscow).8:87–90.
Cohn, Z. 1962. Determination of infection in the peritoneal cavity. I. Response to and fate ofStaphylococcus aureus andStaphlococcus albus in the mouse.Yale J. Biol. Med. 35:12–28.
Kopaniak, M. M., andH. Z. Movat. 1983. Kinetics of acute inflammation induced byEscherichia coli in rabbits. II. The effect of hyperimmunization, complement depletion, and depletion of leukocytes.Am. J. Pathol. 110:13–29.
Skau, T., P.-O. Nystrom, L. Ohman, andO. Stendahl. 1986. The kinetics of peritoneal clearance ofEscherichia coli andBacteroides fragilis and participating defence mechanisms.Arch. Surg. 121:1033–1039.
Devalon, M. L., G. R. Elliott, andW. E. Regelmann. 1987. Oxidative response of human neutrophils, monocytes, and alveolar macrophages induced by unopsonized surface-adherentStaphylococcus aureus.Infect. Immun. 55:2398–2403.
Cheadle, W. G., M. J. Hershman, B. Mays, L. Melton, andH. C. Polk. 1989. Enhancement of survival from murine polymicrobial peritonitis with increase abdominal abscess formation.J. Surg. Res. 47:120–123.
Hansson, L., A. Alwmark, P. Christensen, B. Jeppsson, E. Holst, andS. Bengmark. 1985. Standardized intraabdominal abscess formation with generalized sepsis: Pathophysiology in the rat.Eur. Surg. Res. 17:155–159.
Dr. Tatiana Snastina was a “major scientist” in the Institute of Surgery when this study was done.
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Belotsky, S.M., Guzu, E.V., Karlov, V.A. et al. Wound tissue respiratory burst and local microbial inflammation. Inflammation 14, 663–668 (1990). https://doi.org/10.1007/BF00916369
- Public Health
- Internal Medicine
- Wound Healing
- Wound Infection
- Systemic Response