Advertisement

Journal of Clinical Immunology

, Volume 7, Issue 2, pp 179–184 | Cite as

Preferential human eosinophil chemotactic activity of the platelet-activating factor (PAF) 1-0-hexadecyl-2-acetyl-sn-glyceryl-3-phosphocholine (AGEPC)

  • C. Elliott Sigal
  • Frank H. Valone
  • Michael J. Holtzman
  • Edward J. Goetzl
Short Communication

Abstract

The chemotactic responses of human blood neutrophils and of eosinophils of two different densities, which were resolved by centrifugation on gradients of polyvinylpyrrolidone-coated silica gel (Percoll), were quantified in modified Boyden micropore filter chambers using highly purified synthetic 1-0-hexadecyl-2-acetyl-sn-glyceryl-3-phosphocholine (AGEPC or PAFacether) and leukotriene B4 (LTB4) as stimuli. Maximal chemotactic responses of the densest eosinophils, less dense eosinophils, and neutrophils were evoked by 1 nM, 100 nM, and 1 µM PAFacether, respectively, and by 30–100, 30–100, and 10 nM LTB4. The magnitude of the maximal chemotactic response to PAFacether of the densest eosinophils was significantly greater than that of neutrophils. The eosinophil responses to PAFacether were chemotactic, as distinguished from chemokinetic, and were not influenced by the percentage of contaminating neutrophils. PAFacether is a more potent chemotactic factor for eosinophils than neutrophils and selectively attracts the densest population of human blood eosinophils.

Key words

Eosinophil chemotaxis platelet-activating factor (PAF) allergy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Benveniste J, Camussi J, Polonsky J: Platelet-activating factor.In Monographs in Allergy. Mediators of the Immediate Type Inflammatory Reaction, Vol. 12, KO Rother, Al deWeck (eds). New York, Karger, 1981, pp 138–142Google Scholar
  2. 2.
    Hanahan DJ, Demopoulos CA, Liehr J, Pinckard RN: Identification of platelet activating factor isolated from rabbit basophils as acetyl glyceryl ether phosphorylcholine. J Biol Chem 255:5514–5516, 1980Google Scholar
  3. 3.
    Clark PO, Hanahan DJ, Pinckard RN: Physical and chemical properties of platelet-activating factor obtained from human neutrophils and monocytes and rabbit neutrophils and basophils. Biochim Biophys Acta 628:69–75, 1980Google Scholar
  4. 4.
    Halonen M, Palmer JD, Lohman IC, McManus LM, Pinckard RN: Respiratory and circulatory alterations induced by acetyl glyceryl ether phosphorylcholine, a mediator of IgE anaphylaxis in the rabbit. Am Rev Resp Dis 122:915–924, 1980Google Scholar
  5. 5.
    Darius H, Lefer DJ, Smith JB, Lefer AM: Role of plateletactivating factor-acether in mediating guinea pig anaphylaxis. Science 232:58–60, 1986Google Scholar
  6. 6.
    McManus LM, Hanahan DJ, Demopoulos CA, Pinckard RN: Pathobiology of the intravenous infusion of acetyl glyceryl ether phosphorylcholine (AGEPC), a synthetic platelet-activating factor (PAF), in the rabbit. J Immunol 124:2919–2924, 1980Google Scholar
  7. 7.
    Goetzl EJ, Derian CK, Tauber AI, Valone FH: Novel effects of 1-0-hexadecyl-2-acyl-sn-glycero-3-phosphorylcholine mediators on human leukocyte function: Delineation of the specific roles of the acyl substituents. Biochem Biophys Res Commun 94:881–888, 1980Google Scholar
  8. 8.
    O'Flaherty JT, Lees CJ, Miller CH, McCall CE, Lewis JC, Love SH, Wykle RL: Selective desensitization of neutrophils: Further studies with 1-0-alkyl-sn-glycero-3-phosphocholine analogues. J Immunol 127:731–737, 1981Google Scholar
  9. 9.
    Shaw JO, Pinckard RN, Ferrigni KS, McManus LM, Hanahan DJ: Activation of human neutrophils with 1-0-hexadecyl/octadecyl-2-acetyl-sn-glyceryl-3-phosphorylcholine (platelet activating factor). J Immunol 127:1250–1255, 1981Google Scholar
  10. 10.
    Jouvin-Marche E, Poitevin B, Benveniste J: Platelet-activating factor (PAF-acether), an activator of neutrophil functions. Agents Actions 12:716–720, 1982Google Scholar
  11. 11.
    Valone FH, Goetzl EJ: Enhancement of human polymorphonuclear leukocyte adherence by the phospholipid mediator 1-0-hexadecyl-2-acetyl-sn-glycero-3-phosphorylcholine (AGEPC). Am J Pathol 113:85–89, 1983Google Scholar
  12. 12.
    Gärtner I: Separation of human eosinophils in density gradients of polyvinylpyrrolidone-coated silica gel (Percoll). Immunology 40:133–136, 1980Google Scholar
  13. 13.
    Goetzl EJ, Derian CK, Owens CJ, Valone FH: Modulation of the PMN leukocyte component of hypersensitivity reactions by lipoxygenase products of arachidonic acid.In Advances in Immunopharmacology, J Hadden, L Chedid, P Mullen, F Spreafico (eds). New York, Pergamon Press, 1981, pp 343–353Google Scholar
  14. 14.
    Nagy L, Lee TH, Goetzl EJ, Pickett WC, Kay AB: Complement receptor enhancement and chemotaxis of human neutrophils and eosinophils by leukotrienes and other lipoxygenase products. Clin Exp Immunol 47:541–547, 1982Google Scholar
  15. 15.
    Goetzl EJ, Pickett WC: Novel structural determinants of the human neutrophil chemotactic activity of leukotriene B4. J Exp Med 153:482–487, 1981Google Scholar
  16. 16.
    Lee JY, Chernov T, Goetzl EJ: Characteristics of the epitope of leukotriene B4 recognized by a highly specific mouse monoclonal antibody. Biochem Biophys Res Commun 123:944–950, 1984Google Scholar
  17. 17.
    Goetzl EJ, Austen KF: Structural determinants of the eosinophil chemotactic activity of the acidic tetrapeptides of eosinophil chemotactic factor of anaphylaxis. J Exp Med 144:1424–1437, 1976Google Scholar
  18. 18.
    Wardlaw AJ, Kay AB: PAF-acether is a potent chemotactic factor for human eosinophils. J Allergy Clin Immunol 77:236, 1986Google Scholar
  19. 19.
    Winqvist I, Olofsson T, Olsson I, Persson AM, Hallberg T: Altered density, metabolism, and surface receptors of eosinophils in eosinophilia. Immunology 47:531–539, 1982Google Scholar
  20. 20.
    DeSimone C, Donelli G, Meli D, Rosati F, Sorice F: Human eosinophils and parasitic diseases. II. Characterization of two cell fractions isolated at different densities. Clin Exp Immunol 48:249–255, 1982Google Scholar
  21. 21.
    Roberts RL, Gallin JI: Rapid method for isolation of normal human peripheral blood eosinophils on discontinuous Percoll gradients and comparison with neutrophils. Blood 65:433–440, 1985Google Scholar

Copyright information

© Plenum Publishing Corporation 1987

Authors and Affiliations

  • C. Elliott Sigal
    • 1
  • Frank H. Valone
    • 2
  • Michael J. Holtzman
    • 1
  • Edward J. Goetzl
    • 3
  1. 1.Cardiovascular Research InstituteUniversity of California Medical CenterSan Francisco
  2. 2.Veterans Administration Medical CenterSan Francisco
  3. 3.Howard Hughes Medical Institute and Department of MedicineUniversity of California Medical CenterSan Francisco

Personalised recommendations