Peripheral blood lymphocytes from patients with antibody deficiency diseases (primarily agammaglobulinemia) were examined for the presence of B-lymphocyte subsets defined by surface immunoglobulin isotypes. The patients could be classified into one of four groups based upon the presence or absence of particular isotype-defined subsets. Patients with type I agammaglobulinemia lacked cells bearing surface IgG as well as IgD− Igm+-bearing cells. Type II agammaglobulinemia had unusually large numbers of IgG-bearing cells, representing as many as 50% of the peripheral blood B lymphocytes, while other B-cell subsets were present in normal numbers. Type III agammaglobulinemia had apparently normal numbers of all B-cell subsets. Hyper IgM immunodeficiency lacked cells bearing surface IgG, but did have all three IgD/IgM-bearing B-cell subsets. This classification of patients based upon B-cell subsets present in peripheral blood directly correlates with previous functional studies of B cells from these patients. We suggest that abnormalin vitro function of cells from these patients results from abnormal populations of B cells in peripheral blood, which result from the underlying disease.
This is a preview of subscription content, log in to check access.
Buy single article
Instant access to the full article PDF.
Price includes VAT for USA
Subscribe to journal
Immediate online access to all issues from 2019. Subscription will auto renew annually.
This is the net price. Taxes to be calculated in checkout.
Froland S, Natvig J: Effect of polyspecific rabbit antiimmunoglobulin antisera on human lymphocytes in vitro. Int Arch Allergy Appl Immunol 39:121–132, 1970
Pernis B, Forni L, Amante L: Immunoglobulins as cell receptors. Ann NY Acad Sci 190:420–431, 1971
Rowe D, Hug K, Forni L, Pernis B: Immunoglobulin D as a lymphocyte receptor. J Exp Med 138:965–972, 1973
Abney E, Cooper M, Kearney J, Lawton A, Parkhouse R: Sequential expression of immunoglobulin on developing mouse B lymphocytes. J Immunol 120:2041–2049, 1978
Winchester R, Fu S, Hoffman T, Kunkel H: IgG on lymphocyte surfaces: Technical problems and the significance of a third cell population. J Immunol 114:1210–1212, 1975
Vitetta E, Uhr J: IgD and B cell differentiation. Immunol Rev 37:50–88, 1977
Cooper M, Kearney J, Lawton A, Abney E, Parkhouse R, Preud'homme J, Seligmann M: Generation of immunoglobulin class divesity in B cells: A discussion with emphasis on IgD development. Ann Immunol (Inst Pasteur) 127:573–581, 1976
Goding J, Scott D, Layton J: Genetics, cellular expression, and function of IgD and IgM receptors. Immunol Rev 37:152–186, 1977
Preud'homme J, Griscelli C, Seligmann J: Immunoglobulins on the surface of lymphocytes in fifty patients with primary immunodeficiency diseases. Clin Immunol Immunopathol 1:241–256, 1973
Siegal F, Pernis B, Kunkel H: Lymphocytes in human immunodeficiency states: A study of membrane associated immunoglobulins. Eur J Immunol 1:482–486, 1971
Geha R, Rosen F, Merler F: Heterogeneity of “acquired” or common variable agammaglobulinemia. N Engl J Med 291:1–6, 1974
Cooper M, Lawton A: Circulating B cells in patients with immunodeficiency. Am J Pathol 69:513–528, 1972
Schwaber J, Lazarus H, Rosen F: Bone marrow-derived lymphoid cell lines from patients with agammaglobulinemia. J Clin Invest 62:302–310, 1978
Schwaber J, Lazarus H, Rosen F: Restricted classes of immunoglobulin expressed by a lymphoid cell line derived from an individual with agammaglobulinemia. Proc Natl Acad Sci USA 75:2421–2424, 1978
Ciccimara F, Rosen F, Schneeberger E, Merler E: Failure of heavy chain glycosylation of IgG in some patients with common variable agammaglobulinemia. J Clin Invest 57:1386–1390, 1976
Schwaber J, Lazarus H, Rosen F: IgM restricted production of immunoglobulin by lymphoid cell lines from patients with immunodeficiency with hyper IgM (dysgammaglobulinemia). Clin Immunol Immunopathol 19:91–97, 1981
Geha R, Hyslop N, Alami S, Farah F, Schneeberger E, Rosen F: Hyper immunoglobulin M immunodeficiency (dysgammaglobulinemia). J Clin Invest 64:386–391, 1979
Boyum A: Isolation of lymphocytes, granulocytes, and macrophages. Scand J Immunol 5 (Suppl 5):9–17, 19xx
Winchester R, Fu S: Lymphocyte surface membrane immunoglobulin. Scand J Immunol 5 (Suppl 5):77–82, 19xx
Fuchs S, Sela M: Immunoadsorbents.In Handbook of Experimental Immunology, Vol 1, DM Weir (ed). Oxford, Blackwell Scientific, 1973
Goldman M: Fluorescent Antibody Methods. New York, Academic Press, 1968, pp 97–104
Schwaber J: Agammaglobulinemia in vitro: Cell lines and cell hybrids.In Primary Immunodeficiencies, M Seligmann, W Hitzig (ed). Amsterdam, Elsevier/North-Holland, 1980, pp 49–57
Kincade P, Lawton A, Bockman D, Cooper M: Suppression of immunoglobulin-G synthesis as a result of antibody mediated immunoglobulin M in chickens. Proc Natl Acad Sci USA 67:1918–1925, 1970
Lawton A, Asofsky R, Hylton M, Cooper M: Suppression of immunoglobulin class synthesis in mice. I. Effects of treatment with antibody to u chain. J Exp Med 135:277–297.
Coffman R, Cohn M: The class of surface Ig on virgin and memory B lymphocytes. J Immunol 118:1806–1815, 1977
Schwaber J, Rosen F: Somatic cell hybrid of mouse myeloma cells and B lymphocytes from a patient with agammaglobulinemia: Failure to secrete human Ig. J Immunol 122:1849–1854, 1979
Kanowith-Klein S, Vitetta E, Korn E, Ashman R: Antigen induced changes in the proportion of antigen binding cells expressing IgM, IgG, and IgD receptors. J Immunol 122:2349–2355, 1979
Schwaber J, Klein G, Ernberg I, Rosen A, Lazarus H, Rosen F: Deficiency of Epstein-Barr virus (EBV) receptors on B lymphocytes from certain patients with common varied agammaglobulinemia. J Immunol 124:2191–2196, 1980
About this article
Cite this article
Schwaber, J.F., Rosen, F.S. Isotypes of surface immunoglobulin on B lymphocytes from patients with immune deficiency. J Clin Immunol 2, 30–34 (1982). https://doi.org/10.1007/BF00915975
- B lymphocytes
- immune deficiency
- surface Ig isotypes