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Influence of cationic superoxide generation enhancing protein (SGEP) on phagocytic and intracellular bactericidal activity of human polymorphonuclear cells

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Cationic fraction III from the lysosomes of normal human peripheral blood polymorphonuclear cells (PMNs) was found to contain superoxide generation enhancing protein (SGEP). Herein, we report on the influence of partially purified SGEP obtained from fraction III (subfractions III-5 and III-6), on various phagocytic functions of human PMNs. SGEP markedly enhanced intracellular bactericidal activity of human peripheral PMNs. The enhancement was time and dose dependent. It also reduced adhesiveness of the PMNs. SGEP did not influence chemotaxis, phagocytosis or phagocytic index. These findings are compatible with our original observation regarding superoxide generation enhancement properties of SGEP.

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  1. 1.

    Pruzanski, W., N. S. Ranadive, andS. Saito. 1984. Modulation of phagocytosis and intracellular bactericidal activity of polymorphonuclear and mononuclear cells by cationic proteins from human granulocytes: Alternative pathway of phagocytic enhancement.Inflammation 8:445–456.

  2. 2.

    Stossel, T. P. 1974. Phagocytosis.N. Engl. J. Med. 290:717–723.

  3. 3.

    Pruzanski, W., andS. Saito. 1978. The influence of natural and synthetic cationic substances on phagocytic activity of human polymorphonuclear cells. An alternative pathway of phagocytic enhancement.Exp. Cell Res. 117:1–13.

  4. 4.

    Alam, M., N. S.Ranadive, and W.Pruzanski. 1986. superoxide generation enhancing protein in human polymorphonuclear leukocytes. Book of Abstracts (Abstract 2.56.10) 6th International Congress of Immunology, Toronto. Publ. NRCC No. 25710, Canada. 205.

  5. 5.

    Olsson, I., andP. Venge. 1972. Cationic proteins of human granulocytes. I. Isolation of the cationic proteins from the granules of leukaemic myeloid cells.Scand. J. Haematol. 9:204–214.

  6. 6.

    Olsson, I., andP. Venge. 1974. Cationic proteins of human granulocytes. II. Separation of the cationic proteins of the granules of leukaemic myeloid cells.Blood 44:235–246.

  7. 7.

    MacGregor, R. R. 1976. The effect of anti-inflammatory agents and inflammation on granulocyte adherence: Evidence for regulation by plasma factors.Am. J. Med. 61:597–607.

  8. 8.

    Goldner, M., H. Farkas-Himsley, A. Kormendy, andM. Skinner. 1983. Bacterial phagocytosis monitored by fluorescence and extracellular quenching: Ingestion and intracellular killing.Lab. Med. 14:291–294.

  9. 9.

    Pruzanski, W., S. Saito, andD. W. Nitzan. 1983. The influence of lysostaphin on intracellular kill of microorganisms.J. Lab. Clin. Med. 102:298–305.

  10. 10.

    Nelson, R. D., P. G. Quie, andR. L. Simmons. 1975. Chemotaxis under agarose: A new and simple method for measuring chemotaxis and spontaneous migration of human polymorphonuclear leukocytes and monocytes.J. Immunol. 115:1050–1056.

  11. 11.

    Nelson, R. D., M. P. Bauman, J. L. Gracyk, V. D. Fiegel, andM. J. Herron. 1981. Investigation of Phagocytes in Disease. Churchill Livingstone, Edinburgh. 15–31.

  12. 12.

    Becker, E. 1977. Stimulated neutrophil locomotion: Chemokinesis and chemotaxis.Arch. Pathol. Lab. Med. 101:509–513.

  13. 13.

    Ginsburg, I., andP. G. Quie. 1980. Modulation of human polymorphonuclear leukocyte chemotaxis by leukocyte extracts, bacterial products, inflammatory exudates, and polyelectrolytes.Inflammation 4:301–311.

  14. 14.

    Ginsburg, I., M. N. Sela, A. Morag, Z. Ravid, Z. Duchan, P. P. Thomas, P. Davies, J. Niccols, J. Humes, andR. Bonney. 1981. Role of leukocyte factors and cationic polyelectrolytes in phagocytosis of group A streptococci andCandida albicans by neutrophils, macrophages, fibroblasts and epithelial cells: Modulation by anionic polyelectrolytes in relation to pathogenesis of chronic inflammation.Inflammation 5:301–324.

  15. 15.

    Pruzanski, W., N. S. Ranadive, andS. Saito. 1982. Phagocytic enhancement of granulocytes and monocytes by cationic proteins from human polymorphonuclear leukocytes.In Macrophages and Natural Killer Cells. Ed. S. J. Normann and E. Sorkin, editors. Plenum, New York. 161–164.

  16. 16.

    Nitzan, D. W., W. Pruzanski, S. Saito, andN. Ranadive. 1985. Modulation of locomotor activity of polymorphonuclear cells by cationic substances and cationic lysosomal fractions from human neutrophils.Inflammation 9:375–387.

  17. 17.

    Alam, M., N. S. Ranadive, andW. Pruzanski. 1987. Influence of neutrophil cationic proteins on generation of superoxide by human polymorphonuclear cells during phagocytosis.Inflammation 11:131–142.

  18. 18.

    Venge, P. 1979. Kinetic studies of cell migration in a modified Boyden chamber: Dependence on cell concentration and effects of the chymotrypsin-like cationic protein of human granulocytes.J. Immunol. 122:1180–1184.

  19. 19.

    Johnston, R. B., Jr., B. B. Keele, Jr., H. P. Misra, J. E. Lehmeyer, L. S. Webb, R. L. Baehner, andK. V. Rajagopalan. 1975. The role of superoxide anion generation in phagocytic bactericidal activity. Studies with normal and chronic granulomatous disease leukocytes.J. Clin. Invest. 55:1357–1372.

  20. 20.

    Ganz, T., M. E. Selsted, D. Szklarek, S. S. L. Harwig, K. Daher, D. F. Bainton, andR. I. Lehrer. 1985. Defensins. Natural peptide antibiotics of human neutrophils.J. Clin. Invest. 76:1427–1435.

  21. 21.

    Smith, C. W., J. C. Hollers, R. A. Patrick, andC. Hassett. 1979. Motility and adhesiveness in human neutrophils. Effects of chemotactic factors.J. Clin. Invest. 63:221–229.

  22. 22.

    Lentnek, A. L., A. D. Schreiber, andR. R. MacGregor. 1976. The induction of augmented granulocyte adherence by inflammation. Mediation by a plasma factor.J. Clin. Invest. 57:1098–1103.

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Pruzanski, W., Saito, S., Alam, M. et al. Influence of cationic superoxide generation enhancing protein (SGEP) on phagocytic and intracellular bactericidal activity of human polymorphonuclear cells. Inflammation 12, 99–106 (1988).

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  • Public Health
  • Internal Medicine
  • Superoxide
  • Human Peripheral Blood
  • Polymorphonuclear Cell