Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Macrophage-produced oxygen radical generating activities for polymorphonuclear leukocytes

  • 11 Accesses

  • 4 Citations

Abstract

Guinea pig peritoneal macrophages were stimulated in vitro by bacterial lipopolysaccharide. After incubation, the supernatants of macrophage cultures were collected and tested for O 2 production on guinea pig peritoneal polymorphonuclear leukocytes. The supernatants of macrophage cultures stimulated by lipopolysaccharide had significantly higher levels of O 2 -generating activities in polymorphonuclear leukocytes, and these activities appeared in the macrophage cultures within 2 h after stimulation by lipopolysaccharide However, the supernatants obtained from the nonstimulated cultures could not produce these activities. These activities disappeared with heating or trypsin and were not produced in macrophage cultures by incubation with cycloheximide.

This is a preview of subscription content, log in to check access.

References

  1. 1.

    Allen, R. C., R. L. Stjernholm, andR. H. Steele. 1972. Evidence for the generation of an electronic exicitation state(s) in human polymorphonuclear leukocytes and its participation in bactericidal activity.Biochem. Biophys. Res. Commun. 47:679–684.

  2. 2.

    Babior, B. M., R. S. Kipnes, andJ. T. Curnutte. 1973. Biological defence mechanisms: The production by leukocytes of superoxide, a potential bactericidal agent.J. Clin. Invest. 52:741–744.

  3. 3.

    Root, R. K., J. Metcalf, N. Oshino, andB. Chance. 1975. H2O2 release from human granulocytes during phagocytosis: 1. Documentation, quantittion, and some regulating factors.J. Clin. Invest. 55:945–955.

  4. 4.

    Stevenson, R. D. 1974. Polymorph migration stimulator. A new factor produced by hydrocortisonetreated monocytes.Clin. Exp. Immunol. 17:601–606.

  5. 5.

    Gery, I., andB. H. Waksman. 1972, Potentiation of the T-lymphocyte response to mitogens: II. The cellular source of potentiating mediator(s).J. Exp. Med. 136:143–155.

  6. 6.

    Mizel, S. B., andD. L. Rosenstreich, 1979. Regulation of lymphocyteactivating factor (LAF) production and secretion in P388D1 cells: Identification of high molecular weight precursors of LAF.J. Immunol 122:2173–2179.

  7. 7.

    Leibovich, S. J., andR. Ross. 1976. A macrophage-dependent factor that stimulates the proliferation of fibroblasts in vitro.Am. J. Pathol. 84:501–514.

  8. 8.

    Wahl, S. M., L. M. Wahl, J. B. Mccarthy, L. Chedid, andS. E. Mergenhagen. 1979. Macrophage activation by mycobacterial water soluble compounds and synthetic muramyl dipeptide.J. Immunol. 122:2226–2231.

  9. 9.

    Tsukamoto, Y., W. E. Helsel, andS. M. Wahl. 1981. Macrophage production of fibronectin, a chemoattractant for fibroblasts.J. Immunol. 127:673–678.

  10. 10.

    Wahl, S. M., L. C. Altman, J. J. Oppenheim, andS. E. Mergenhagen. 1974. In vitro studies of a chemotactic lymphokine in the guinea pig.Int. Arch. Allergy Appl. Immunol. 46:768:784.

  11. 11.

    Pick, E., andD. Mizel. 1981. Rapid microassays for the measurement of superoxide and hydrogen peroxide production by macrophages in culture using an automatic enzyme immunoassay reader.J. Immunol. Methods 46:211–226.

  12. 12.

    Van Epps, D. E., andM. L. Garcia. 1980. Enhancement of neutrophil function as a result of prior exposure to chemotactic factor.J. Clin. Invest. 66:167–175.

  13. 13.

    Ward, P. A., M. C. Sulavik, andK. J. Johnson. 1985. Activated rat neutrophils: Correlation of arachidonate products with enzyme secretion but not with O 2 generation.Am. J. Pathol. 120:112–120.

  14. 14.

    Yagawa, K., M. Kaku, Y. Ichinose, Y. Aida, andA. Tomoda. 1985. Fc receptormediated desensitization of superoxide (O 2 ) generation response of guine-a-pig macrophages and polymorphonuclear leucocytes.Immunology 55:629–637.

  15. 15.

    Babior, G. L., R. E. Rosin, B. J. McMurrich. 1981. Arrangement of the respiratory burst oxidase in the plasma membrane of the neutrophil.J. Clin. Invest. 67:1724–1728.

  16. 16.

    Kaku, M., K. Yagawa, S. Nagao, andA. Tanaka. 1983. Enhanced superoxide anion release from phagocytes by muramyl dipeptide or lipopolysaccharide.Infect. Immun. 39:559–564.

  17. 17.

    Pennington, J. E., T. H. Rossing, L. W. Boerth, andT. H. Lee. 1985. Isolation and partial characterization of a human alveolar macrophage-derived neutrophil-activating factor.J. Clin. Invest. 75:1230–1237.

  18. 18.

    Yoshimura, T., K. Matsushima, S. Tanaka, E, A. Robinson, E. Appella, J. J. Oppenheim, andE. J. Leonard. 1987. Purification of a human monocyte-derived neutrophil chemotactic factor that has peptide sequence similarity to other host defense cytokines.Proc. Natl. Acad. Sci. U.S.A. 84:9233–9237.

  19. 19.

    Walz, A., P. Peveri, H. Aschauer, andM, Baggiolini. 1987. Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes.Biochem. Blophys. Res. Commun. 149:755–761.

Download references

Author information

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Tsukamoto, Y., Fukutani, S., Nakatani, M. et al. Macrophage-produced oxygen radical generating activities for polymorphonuclear leukocytes. Inflammation 13, 259–266 (1989). https://doi.org/10.1007/BF00914393

Download citation

Keywords

  • Oxygen
  • Public Health
  • Internal Medicine
  • Trypsin
  • Generate Activity