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Chemotactic competence of neutrophils from neonatal calves

Functional comparison with neutrophils from adult cattle

Abstract

Neonates demonstrate an increased susceptibility to infection. Defects in locomotory functions of newborn neutrophils may play a crucial role in this context. We therefore compared the migratory response of newborn (N-PMN) and adult (APMN) bovine neutrophils in a microwell filter assay. Stimulation with four different endotoxins (E. coli O128 B∶:4 and O55 B∶5;S. abortus equi; S. typhimurium), with zymosan-activated plasma (ZAP) and with C5a induced dose-dependent migration of A-PMNs and N-PMNs. Migration of unstimulated cells and of cells stimulated with diluted ZAP or C5a was higher (P < 0.05) in N-PMNs. Migration of A- and N-PMNs towards C5a was inhibited (P < 0.001) by preincubation with either a steroidal (122μM flumethasone) or nonsteroidal (3.3μM phenylbutazone) antiinflammatory drug. Migratory responses of N-PMNs were inhibited less by SAIDs than were responses of A-PMNs (P < 0.05); indeed dexamethasone slightly enhanced N-PMN responses towards C5a, and 510μM flunixin meglumine enhanced C5a-induced migration in both age groups. Endotoxins fromE. coli O55:B4,S. abortus equi, and S. typhimurium induced a higher rate of migration (P < 0.05) in N-PMNs. In contrast to the above findings, measurement of the maximal distance of migration by the leading-front method did not reveal age-related differences. Migration speed of PMNs was lower after stimulation with C5a than with ZAP, but could be restored partly by adding human vitamin D-binding protein (Gc-globulin). The demonstrated hyperirritability of bovine N-PMNs represents a major functional difference to neonatal neutrophils from other species, including man. It may additionally be related to altered PMN functions and neonatal disease susceptibility.

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Zwahlen, R.D., Roth, D.R. Chemotactic competence of neutrophils from neonatal calves. Inflammation 14, 109–123 (1990). https://doi.org/10.1007/BF00914034

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Keywords

  • Phenylbutazone
  • Unstimulated Cell
  • Meglumine
  • Migration Speed
  • Locomotory Function